PXD077149-1

PXD077149 is an original dataset announced via ProteomeXchange.

Title	Enhancing the Recovery of Antioxidant Compounds from Microalgae-Cyanobacteria Consortia through Alcalase Hydrolysis: a Focus on Bioactive Peptides
Description	Microalgae and cyanobacteria represent an emerging and sustainable source of bioactive compounds for the food, cosmeceutical, and pharmaceutical sectors. In this study, the potential of two microalgae-cyanobacteria consortia, consortium 1 (C1) consisting of Chlorella vulgaris and Arthrospira platensis, and consortium 2 (C2) consisting of Kamptonema sp., Nannochloropsis oculata, Tetraselmis suecica, and Chlorella vulgaris, as a source of bioactive peptides was evaluated. Firstly, protein extraction from both biomasses was optimized by testing different protein solubilization and precipitation pHs, with pH 10 and pH 5 providing the best results in terms of protein recovery in both cases. Selected protein extracts, with protein contents of 28.50 ± 2.69 % (C1) and 8.46 ± 0.45 % (C2), were further hydrolyzed with Alcalase, evaluating the impact of the incubation time on peptide release and the antioxidant capacity of hydrolysates. 1 h of hydrolysis proved to be enough for antioxidant capacity increase. In addition, in silico hydrolysis with Alcalase of the proteins identified in C1 and C2 was evaluated, assessing the potential bioactivity of the peptides produced, more specifically their antioxidant capacity. Our findings demonstrate that both microalgae-cyanobacteria consortia are valuable sources of bioactive compounds with antioxidant capacity, with potential interest as functional ingredients for the food, cosmeceutical, and pharmaceutical industries.
HostingRepository	PRIDE
AnnounceDate	2026-05-21
AnnouncementXML	Submission_2026-05-21_06:07:45.830.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD077149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Blanca Pardo de Donlebún
SpeciesList	scientific name: Nannochloropsis oculata; NCBI TaxID: NCBITaxon:43925; scientific name: Tetraselmis suecica; NCBI TaxID: NEWT:270643; scientific name: Chlorella vulgaris; NCBI TaxID: NCBITaxon:3077; scientific name: Kamptonema sp.; NCBI TaxID: NCBITaxon:2719036;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-14 12:15:47	ID requested
⏵ 1	2026-05-21 06:07:46	announced

10.6019/PXD077149;

10.3390/MD24050184;

submitter keyword: None

Blanca Hernández-Ledesma
contact affiliation	Department of Bioactivity and Food Analysis, Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain
contact email	b.hernandez@csic.es
lab head
Blanca Pardo de Donlebún
contact affiliation	Consejo Superior de Investigaciones Científicas (CSIC)
contact email	blanca.pardo@csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD077149

PRIDE project URI

• PRIDE
  1. PXD077149
     1. Label: PRIDE project
     2. Name: Enhancing the Recovery of Antioxidant Compounds from Microalgae-Cyanobacteria Consortia through Alcalase Hydrolysis: a Focus on Bioactive Peptides