PXD077080 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Adaptation of an immunoaffinity chromatography protocol for the isolation of the cell line immunopeptides |
| Description | BACKGROUND. For developing targeted immunotherapy against ovarian adenocarcinoma, direct identification of peptides presented by major histocompatibility complex class I (HLA I) on the surface of tumor cells can be utilized. However, standard immunopeptidomics protocols may vary substantially in their efficiency of immunopeptidome isolation depending on lysis conditions. Optimizing detergents for immunoaffinity purification of complexes enhances analytical sensitivity, expands the repertoire of identified ligands, and increases the likelihood of detecting clinically relevant peptides suitable for personalized immunotherapeutic approaches.
AIM. To evaluate the effect of various detergents on the efficiency of identification of peptide ligands of HLA I complexes.
METHODS. Immunopeptidome from cell lines were isolated using immunoaffinity chromatography and analyzed by liquid chromatography-mass spectrometry.
RESULTS. In this study, we tested an affinity chromatography-based immunopeptidome isolation protocol, comparing various detergents (CHAPS, NP-40, SOD, and Triton X-100) for cell lysis, and observed no statistically significant differences in the number of identified peptides.
CONCLUSION. Each of the four tested detergents provides identification of unique sets of peptides. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-15 |
| AnnouncementXML | Submission_2026-04-15_09:04:51.685.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD077080 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Georgij Arapidi |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-13 08:31:05 | ID requested | |
| 1 | 2026-04-15 09:01:01 | announced | |
| ⏵ 2 | 2026-04-15 09:04:52 | announced | 2026-04-15: Updated project metadata. |
Publication List
Keyword List
Contact List
| Georgij Arapidi |
|---|
| contact affiliation | Head of System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| lab head | |
| Georgij Arapidi |
|---|
| contact affiliation | System Biology Lab. at Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency |
| contact email | arapidi@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077080
- Label: PRIDE project
- Name: Adaptation of an immunoaffinity chromatography protocol for the isolation of the cell line immunopeptides
