PXD077076 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The toxin from the PemIK-Sa1 toxin-antitoxin system decreases Staphylococcus aureus resistance to antibiotics |
| Description | Toxin-antitoxin (TA) systems are bacteria-specific components involved in maintaining mobile genetic elements that may carry antibiotic resistance determinants (ARDs), among other functions. PemIK-Sa1 is a plasmid-encoded TA system found in Staphylococcus aureus, a dangerous pathogen affecting humans and animals. The PemK-Sa1 toxin is a sequence-specific ribonuclease inhibited by its cognate antitoxin, PemI-Sa1. Using recombinant multi-drug-resistant strains, we demonstrated that expression of the toxin reduces resistance to antibiotics from various classes, including β-lactams, fluoroquinolones, aminoglycosides, and chloramphenicol. Transcriptomics revealed that, in addition to downregulation of genes encoding ARDs, nearly one-third of the staphylococcal transcriptome is altered. Furthermore, proteomic analysis revealed that toxin expression results in the downregulation of several proteins involved in nucleotide biosynthesis, carbohydrate metabolism, and energy production. This indicates parallel mechanisms in which the toxin not only degrades ARD transcripts, thereby directly impacting their function, but also alters the expression of genes encoding proteins crucial to essential cell processes. The ability to reduce resistance to a broad range of antibiotics makes the PemK-Sa1 toxin a promising tool for combating multidrug-resistant bacteria through the artificial activation of the PemIK-Sa1 TA system. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-16 |
| AnnouncementXML | Submission_2026-08-16_07:21:19.796.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Piotr Suder |
| SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: NEWT:1280; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | orbitrap instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-13 07:43:37 | ID requested | |
| ⏵ 1 | 2026-08-16 07:21:20 | announced | |
Publication List
Keyword List
| submitter keyword: PemIK-Sal, toxin-antitoxin system, S.aureus |
Contact List
| Piotr Suder |
|---|
| contact affiliation | Biochemistry and Neurobiology Group, Department of Analytical Chemistry and Biochemistry, Faculty of Material Sciences and Ceramics, AGH University of Krakow, Mickiewicza 30 avenue, 30-059 Krakow, Poland |
| contact email | psuder@agh.edu.pl |
| lab head | |
| Piotr Suder |
|---|
| contact affiliation | AGH University of Krakow, Department of Materials Sciences and Ceramics, Mickiewicza 30, 30-059 Krakow, Poland |
| contact email | psuder@agh.edu.pl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077076
- Label: PRIDE project
- Name: The toxin from the PemIK-Sa1 toxin-antitoxin system decreases Staphylococcus aureus resistance to antibiotics
