PXD077067-1

PXD077067 is an original dataset announced via ProteomeXchange.

Title	DIA-Based Proteomics Delineates an Ordered Pathological Axis of Endothelial Barrier Disruption and Immunothrombosis in Acute Lung
Description	The core pathophysiological mechanism of acute lung injury (ALI) is a vicious cycle of inflammation, endothelial barrier disruption, and coagulation abnormalities. Conventional single-molecule studies cannot elucidate these mechanisms at the network-regulation level. This study combined proteomics with transcriptomic validation and identified 1,596 differentially expressed proteins in a lipopolysaccharide-induced ALI model. The proteins were enriched in immune inflammation, cytoskeletal regulation, and multiple key signaling pathways. Further validation confirmed that ALI pathology begins with Casp1/Tlr2-mediated neutrophil extracellular trap formation. The process progresses via vascular glycocalyx degradation, protein homeostasis disruption, and endothelial cell programmed death. Ultimately, these changes lead to microcirculatory thrombosis, forming an ordered amplified pathogenic axis. The tyrosine kinase receptor Met and the heme metabolism–epigenetic regulation network are key regulatory nodes. These findings systematically shed light on the multilevel networked regulatory mechanisms of ALI, providing a strong theoretical foundation for targeting pathological vicious cycles and developing novel intervention strategies.
HostingRepository	iProX
AnnounceDate	2026-04-13
AnnouncementXML	Submission_2026-04-13_01:12:09.347.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Feifei Ma
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-13 01:10:43	ID requested
⏵ 1	2026-04-13 01:12:09	announced

Dataset with its publication pending

submitter keyword: acute lung injury, proteomics, differentially expressed proteins, endothelial barrier, microcirculatory thrombosis, pathological cascade

Feifei Ma
contact affiliation	Gansu Agricultural University
contact email	1046617673@qq.com
lab head
Feifei Ma
contact affiliation	Gansu Agricultural University
contact email	1046617673@qq.com
dataset submitter

iProX dataset URI