PXD077030 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networks |
| Description | Phosphatidylinositol-5-phosphate 4-kinases (PI5P4Ks) have emerged as candidate drug targets in cancer, neurological, inflammatory, and infectious diseases. Although their canonical function is to phosphorylate PI(5)P to generate PI(4,5)P2, growing evidence points to additional catalytic-independent roles, but how these functions are organized within protein interaction networks remains unclear. Here, we use proximity-dependent biotin identification (BioID) in HeLa cells to map isoform-resolved interactomes of human PI5P4Kα, PI5P4Kβ, and PI5P4Kγ. This approach captures PI5P4K-proximal proteins in intact cells and reveals interaction networks positioning these kinases within trafficking-associated signaling modules. Importantly, BioID analysis indicates that PI5P4Kγ has the most extensive set of proximal interactors among the three PI5P4Ks, consistent with its comparatively low catalytic activity and a prominent scaffold-like function. Among PI5P4Kγ-enriched partners, we highlight the endosomal cargo adaptor SNX17 as a proximal interactor that links PI5P4Kγ to β1-integrin recycling and to cell migration and invasion. By providing a proximity map for all three PI5P4Ks, this study offers a framework to help define contexts in which targeted protein degradation may offer advantages over catalytic inhibition and provides a resource for future mechanistic studies on these phosphoinositide kinases. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-05-05 |
| AnnouncementXML | Submission_2026-05-04_19:35:36.427.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rabi Murad |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-10 22:07:44 | ID requested | |
| ⏵ 1 | 2026-05-04 19:35:37 | announced | |
Publication List
Keyword List
| submitter keyword: HeLa, PI5P4Ks, BioID |
Contact List
| Brooke Emerling |
|---|
| contact affiliation | Cancer Metabolism and Microenvironment Program, NCI-Designated Cancer Center, Sanford Burnham Prebys Medical Discovery Institute |
| contact email | bemerling@sbpdiscovery.org |
| lab head | |
| Rabi Murad |
|---|
| contact affiliation | Sanford Burnham Prebys Medical Discovery Institute |
| contact email | rmurad.geo03@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD077030
- Label: PRIDE project
- Name: A proximity-labeling map of PI5P4K phosphoinositide kinases interaction networks
