PXD076861-1

PXD076861 is an original dataset announced via ProteomeXchange.

Title	Native MS and intact-protein MS datasets for NreB and NreB_PAS supporting the study “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”
Description	This ProteomeXchange dataset contains native mass spectrometry and intact-protein mass spectrometry data generated in support of the manuscript “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”. The dataset includes intact-protein MS measurements of purified His_NreB and His_NreB_PAS for molecular-mass verification, as well as native MS measurements of NreB_PAS under untreated, GSH-treated, GSH plus NaHS-treated, and pH-dependent conditions. These data were used to characterize Fe-S cluster-associated adduct states and to compare the distributions of species detected under different conditions. Raw data acquired on a Bruker Impact HD mass spectrometer and the corresponding deconvolution outputs generated using Bruker DataAnalysis 4.2 are included together with a deconvolution summary table.
HostingRepository	PRIDE
AnnounceDate	2026-04-15
AnnouncementXML	Submission_2026-04-15_05:15:35.055.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	chao tang
SpeciesList	scientific name: Bacillus licheniformis DSM 13 = ATCC 14580; NCBI TaxID: NEWT:279010;
ModificationList	No PTMs are included in the dataset
Instrument	impact

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-10 02:19:07	ID requested
⏵ 1	2026-04-15 05:15:35	announced

Dataset with its publication pending

submitter keyword: NreB_PAS, GSH,NreB, Fe-S cluster, NaHS, native MS, sulfide sensing, Bruker Impact HD, [2Fe-2S], intact protein MS

Yongzhen Xia
contact affiliation	State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, People’s Republic of China
contact email	xiayongzhen2002@sdu.edu.cn
lab head
chao tang
contact affiliation	Shandong university
contact email	1638254392@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076861

PRIDE project URI

• PRIDE
  1. PXD076861
     1. Label: PRIDE project
     2. Name: Native MS and intact-protein MS datasets for NreB and NreB_PAS supporting the study “A labile sulfur ligand in a three-cysteine-coordinated [2Fe-2S] cluster mediates sulfide sensing in NreB”