PXD076800 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | The development of activity-based mannanase probes |
| Description | A Biotin-Man-manno-cyclophellitol activity-based probe (ABP) was used to pull-down putative mannanases from the secretome of the parental strain of A. niger MA234.1 grown for at least 72h after transfer on konjac flour (kF). AnManA was successfully enriched in the secretome. Total protein identification of the same secretome did not reveal the presence of another well characterized mannanase form A. niger: AnMan26A Both recombinant AnManA and AnMan26A were expressed in A. niger and purified. The recombinant proteins were labelled with either a Man-manno-cyclophellitol or a Man-man-manno-cyclophellitol probe. The m/z of the complex between the probe and the tryptic peptide containing the active-site nucleophile of the two mannanases was successfully identified. This way the mechanism-based activity of the developed mannanase ABPs was confirmed. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-17 |
| AnnouncementXML | Submission_2026-07-16_23:12:03.362.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Bogdan Florea |
| SpeciesList | scientific name: Fungi; NCBI TaxID: NEWT:4751; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-09 13:01:04 | ID requested | |
| ⏵ 1 | 2026-07-16 23:12:03 | announced | |
Publication List
Keyword List
| submitter keyword: cyclophellitol,Aspergillus niger, endo-mannanases, activity-based probes |
Contact List
| Bogdan I. Florea |
|---|
| contact affiliation | Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands |
| contact email | b.florea@chem.leidenuniv.nl |
| lab head | |
| Bogdan Florea |
|---|
| contact affiliation | Leiden Institute for Chemistry |
| contact email | b.florea@chem.leidenuniv.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD076800 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076800
- Label: PRIDE project
- Name: The development of activity-based mannanase probes
