PXD076795 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PSMB4 pulldown ID with PSMD5 overexpression |
| Description | For PSMD5 over expression experiment, PSMB4-tagged cells were transfected in either 6-well plates (Cat. No. 3506; Costar) or 10 cm dishes using 1 µg or 5 µg of HA-PSMD5 or HA-PSMD5ΔCT, respectively. All the transfections were performed with Lipofectamine 3000 (Cat. No. L3000015; Thermo Fisher Scientific) in Opti-MEM reduced serum medium (Cat. No. 31985070; Life Technologies), following the manufacturer’s protocol. Cells were incubated at 37 °C in a humidified atmosphere containing 5 % CO₂ for 48 h before harvesting. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-16 |
| AnnouncementXML | Submission_2026-07-16_08:41:39.507.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Kitaik Lee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine residue |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-09 12:58:49 | ID requested | |
| ⏵ 1 | 2026-07-16 08:41:40 | announced | |
Publication List
Keyword List
| submitter keyword: psmd5,proteasome |
Contact List
| Francis J.O'Reilly |
|---|
| contact affiliation | Center for Structural Biology, Center for Cancer Research, National Cancer Institute (NCI), National Institutes of Health, Frederick, MD 21702-1201, USA Center of Excellence for Data Driven Discovery, Department of Structural Biology, St Jude Children’s Research Hospital, Memphis, Tennessee 38105, United States |
| contact email | francis.oreilly@stjude.org |
| lab head | |
| Kitaik Lee |
|---|
| contact affiliation | Center of Excellence for Data Driven Discovery, Department of Structural Biology, St Jude Children’s Research Hospital, Memphis, Tennessee 38105, United States |
| contact email | kitaik.lee@stjude.org |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD076795 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076795
- Label: PRIDE project
- Name: PSMB4 pulldown ID with PSMD5 overexpression
