PXD076790-1

PXD076790 is an original dataset announced via ProteomeXchange.

Title	Loss of mitochondrial complex II causes severe respiratory chain dysfunction
Description	Mitochondrial complex II (succinate dehydrogenase, SDH) links the tricarboxylic acid cycle to the respiratory chain. To investigate how disruption of complex II affects mitochondrial respiratory organization, we generated cell lines lacking either SDHA or SDHB and systematically characterized the activity and organization of mitochondrial respiratory complexes, as well as mitochondrial architecture. Loss of either subunit abolished complex II activity and induced extensive remodeling of the respiratory chain, including a marked reduction in the abundance of the respirasome and other complex I–containing supercomplexes. These changes were accompanied by impaired mitochondrial respiration, altered mitochondrial morphology and a pronounced reduction in cristae number. Quantitative proteomic analysis of respiratory chain complexes revealed coordinated changes in the abundance and organization of OXPHOS components. Together, these findings demonstrate that disruption of complex II drives large-scale remodeling of respiratory chain organization and mitochondrial architecture
HostingRepository	PRIDE
AnnounceDate	2026-07-03
AnnouncementXML	Submission_2026-07-03_00:01:14.441.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enrique Calvo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-09 12:56:55	ID requested
⏵ 1	2026-07-03 00:01:14	announced

Dataset with its publication pending

submitter keyword: respiratory chain,complex II

Enrique Calvo
contact affiliation	CNIC
contact email	ecalvo@cnic.es
lab head
Enrique Calvo
contact affiliation	CNIC
contact email	ecalvo@cnic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD076790

PRIDE project URI

• PRIDE
  1. PXD076790
     1. Label: PRIDE project
     2. Name: Loss of mitochondrial complex II causes severe respiratory chain dysfunction