PXD076789-1

PXD076789 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics of tau from tau knockout mice expressing tau in hippocampal neurons during spatial reference learning
Description	This dataset describes the mass spectrometric identification of phosphorylation sites on tau protein isolated from the hippocampus of tau knockout (tau‑/‑) mice during spatial reference learning. Tau expression was selectively reintroduced into hippocampal neurons using adeno‑associated viral (AAV) vectors to enable controlled investigation of tau phosphorylation under defined behavioural conditions. Tau‑/‑ mice received bilateral injections into the dorsal and ventral hippocampus with either AAV‑syn1‑d2tTA‑tau or the control vector AAV‑syn1‑d2tTA‑eGFP. Following postoperative recovery, mice were trained in a spatial reference learning paradigm (day 2 of Morris water maze). Temporal control of tau or eGFP expression was achieved using the doxycycline-regulated d2tTA system, with induction on day 2 of training. Hippocampal tissue was collected at this time point for biochemical analysis. Tau protein was immunoprecipitated from hippocampal lysates and subjected to bottom‑up proteomic analysis. Immunoprecipitated material was reduced, alkylated, and digested with trypsin. Phosphopeptides were subsequently enriched using titanium dioxide (TiO₂) affinity chromatography. Enriched phosphopeptides underwent buffer exchange, vacuum centrifugation, and resuspension prior to liquid chromatography–tandem mass spectrometry (LC‑MS/MS) analysis.
HostingRepository	PRIDE
AnnounceDate	2026-04-10
AnnouncementXML	Submission_2026-04-09_17:30:29.361.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Arne Ittner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	4800 Plus MALDI TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-09 12:56:45	ID requested
⏵ 1	2026-04-09 17:30:29	announced

Dataset with its publication pending

submitter keyword: hippocampus, spatial reference learning,Tau, phosphoproteomics

Arne Ittner
contact affiliation	Flinders Health and Medical Research Institute, College of Medicine and Public Health, Flinders University, Adelaide, Australia
contact email	arne.ittner@flinders.edu.au
lab head
Arne Ittner
contact affiliation	Flinders University
contact email	arne.ittner@flinders.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076789
     1. Label: PRIDE project
     2. Name: Phosphoproteomics of tau from tau knockout mice expressing tau in hippocampal neurons during spatial reference learning