PXD076745-1

PXD076745 is an original dataset announced via ProteomeXchange.

Title	Anti-Flag AP-MS of cytoplasmic 3×Flag-importin α1 complexes in HeLa S3 cells
Description	To identify binding partners of importin α1 in the cytoplasm, co-immunoprecipitation using an anti-Flag antibody in the cytoplasmic fraction of 3×Flag–importin α1-inducible HeLaS3 cells.
HostingRepository	PRIDE
AnnounceDate	2026-04-22
AnnouncementXML	Submission_2026-04-21_18:30:31.414.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yutaka Ogawa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-08 02:18:30	ID requested
⏵ 1	2026-04-21 18:30:31	announced

10.1242/JCS.264517;

submitter keyword: nuclear localization signal, HeLaS3,Importin, NLS

Naoko Imamoto
contact affiliation	Cellular Dynamics Laboratory, RIKEN Cluster for Pioneering Research (CPR),Saitama 3510198, Japan. Present address:Jikei University of Health Care Sciences, Graduate School of Medical Safety Management, Osaka, Japan.
contact email	n-imamoto@juhs.ac.jp
lab head
Yutaka Ogawa
contact affiliation	Riken CSRS
contact email	yogawa@riken.jp
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD076745

PRIDE project URI

• PRIDE
  1. PXD076745
     1. Label: PRIDE project
     2. Name: Anti-Flag AP-MS of cytoplasmic 3×Flag-importin α1 complexes in HeLa S3 cells