PXD076718 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of HoxB8-derived macrophages in which the Slc31a1 or Atp7a genes were inactivated using CRISPR/Cas9 |
| Description | This project is part of a study aimed at elucidating how Mycobacterium tuberculosis responds to physiologically relevant copper levels during macrophage infection. Here, we conducted a proteomic analysis of macrophages derived from immortalized HoxB8 hematopoietic precursors, in which the Slc31a1 (encoding the Copper transporter 1 or CTR1) or Atp7a (encoding the Copper-transporting ATPase 1) genes were inactivated using CRISPR/Cas9. A luciferase-targeting sgRNA (Luc) served as a control. To validate gene inactivation and evaluate its impact on the macrophage proteome, we systematically compared the proteomic profiles of control (Luc) macrophages with those deficient in CTR1 or ATP7A. Each phenotype was analyzed using four biological replicates, ensuring robust statistical power for downstream analyses. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-25 |
| AnnouncementXML | Submission_2026-08-25_03:32:44.189.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Anne Gonzalez de Peredo |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-04-07 18:37:27 | ID requested | |
| ⏵ 1 | 2026-08-25 03:32:44 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: copper receptors, Atp7a, Slc31a1,Macrophages |
Contact List
| Odile Schiltz |
|---|
| contact affiliation | Institute of Pharmacology and Structural Biology, IPBS, CNRS, Toulouse, France |
| contact email | gonzalez@ipbs.fr |
| lab head | |
| Anne Gonzalez de Peredo |
|---|
| contact affiliation | CNRS/IPBS |
| contact email | gonzalez@ipbs.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD076718 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076718
- Label: PRIDE project
- Name: Proteomic analysis of HoxB8-derived macrophages in which the Slc31a1 or Atp7a genes were inactivated using CRISPR/Cas9
