PXD076700-1

PXD076700 is an original dataset announced via ProteomeXchange.

Title	Microbiota-induced fatty acid synthesis facilitates intestinal infection and immune-mediated damage in Drosophila
Description	The role of the microbiota in facilitating infections is increasingly recognized, though the underlying mechanisms remain under investigation. In this study, we demonstrate that the gut microbiome, particularly Lactiplantibacillus plantarum, promotes enteric infection in Drosophila melanogaster by reshaping host physiology and immune responses. Upon gut colonization, L. plantarum enhances fatty acid (FA) production in the gut. Reducing FA synthesis improves host survival, and pathogen mutants deficient in FA utilization exhibit reduced virulence. FAs support pathogen growth, increase virulence, and enhance resistance to antimicrobial effectors, ultimately facilitating pathogen persistence in the gut. Persisting pathogens consequently overstimulate the immune system, leading to excessive production of antimicrobial peptides (AMPs). Flies lacking AMPs, particularly Metchnikowin and Attacin D mutants, show better survival during infection, implicating AMPs in immunopathology. Our findings identify a mechanism whereby microbiota-induced release of host FAs stimulates pathogen virulence and persistence, ultimately driving AMP-mediated immunopathology.
HostingRepository	PRIDE
AnnounceDate	2026-08-17
AnnouncementXML	Submission_2026-08-17_13:17:05.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kathirvel Alagesan
SpeciesList	scientific name: Pseudomonas entomophila L48; NCBI TaxID: NEWT:384676;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-07 09:28:22	ID requested
⏵ 1	2026-08-17 13:17:06	announced

Dataset with its publication pending

submitter keyword: microbiota, fatty acids, virulence, Lactiplantibacillus plantarum, Drosophila, host-microbe interactions, antimicrobial peptides, immunopathology

Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens Charitéplatz 1 | D-10117 Berlin | Germany
contact email	alagesan@mpusp.mpg.de
lab head
Kathirvel Alagesan
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	alagesan@mpusp.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD076700

PRIDE project URI

• PRIDE
  1. PXD076700
     1. Label: PRIDE project
     2. Name: Microbiota-induced fatty acid synthesis facilitates intestinal infection and immune-mediated damage in Drosophila