PXD076697-1

PXD076697 is an original dataset announced via ProteomeXchange.

Title	Sperm proteome modifications during the post-testicular maturation in Apis mellifera drones
Description	Despite the biological importance of bees, no information of the sperm proteome during the completion of SV filling and the attainment of full sperm maturation in A. mellifera. Despite not finding differences in seminal vesicle sperm motility and viability between mature and immature drones, significant variations were identified at proteome. The results revealed a biphasic pattern characterized by a massive loss of proteins—particularly those involved in protein translation (ribonucleoprotein complexes) or post-translational modification processes (Golgi apparatus), electron transport chain (ETC) complexes and proteasome—and the preservation of a highly stable subset comprising various metabolic pathways and the flagellar structure in spermatozoa during drone maturation. This suggests the presence of tightly regulated quality-control mechanisms that maintain a precise balance between protein conservation with selective degradation. Moreover, the observed modulation of energy-related proteins, particularly the decreasing of complexes I, III and V of the ETC, may represent an adaptive strategy to enhance cellular longevity.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_05:17:33.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luz Valero
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-04-07 09:16:50	ID requested
⏵ 1	2026-09-07 05:17:33	announced

Dataset with its publication pending

submitter keyword: bees, diaPASEF,sperm proteome

Miguel Ángel Silvestre
contact affiliation	Departamento: Biología Celular, Biología Funcional y Antropología Física Universitat de Valencia (Spain)
contact email	miguel.silvestre@uv.es
lab head
Luz Valero
contact affiliation	Universitat de Valencia
contact email	mluz.valero@uv.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076697

PRIDE project URI

• PRIDE
  1. PXD076697
     1. Label: PRIDE project
     2. Name: Sperm proteome modifications during the post-testicular maturation in Apis mellifera drones