⮝ Full datasets listing
PXD076529-1
PXD076529 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic Profiling of Lipofuscin-Stratified Microglia from Aged Grn Knockout Mice |
| Description | By profiling transcriptomic signatures in aged Grn KO mouse brains, we identified a subpopulation of microglia with high expression of GPNMB and marked lipofuscinosis. To further characterize this population, we isolated lipofuscin "high" and "low" microglia (CD11b+/CD45+) by FACS from the brains of aged (20-month-old) WT and Grn KO mice (n = 4-6/group) and investigated the proteome. Proteomics confirmed an increase in Grn KO specific markers identified by transcriptomics, such as GPNMB. Additionally, proteins belonging to the coordinated lysosomal expression and regulation (CLEAR) network were upregulated, suggesting lysosomal dysfunction in these cells. Finally, myelin-specific proteins were elevated in GrnKO microglia, and some were further elevated in lipofuscin "high" cells, consistent with previous reports of myelin debris accumulating in Grn KO mouse and GRN-FTD patient brain. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-07-17 |
| AnnouncementXML | Submission_2026-07-17_13:29:10.605.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Thomas Sandmann |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-04-02 17:36:48 | ID requested | |
| ⏵ 1 | 2026-07-17 13:29:11 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Progranulin, Lipofuscin, microglia, DatasetType:Proteomics |
Contact List
| Thomas Sandmann | |
|---|---|
| contact affiliation | Denali Therapeutics Inc |
| contact email | sandmann@dnli.com |
| lab head | |
| Thomas Sandmann | |
| contact affiliation | Denali Therapeutics |
| contact email | sandmann.t@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101344/ |
