PXD076436-1

PXD076436 is an original dataset announced via ProteomeXchange.

Title	Oocyte polarity is established independently of the Balbiani body
Description	In most animals, oocyte polarity establishes the embryonic body plan by asymmetrically localizing axis-determining transcripts along the animal-vegetal axis. These transcripts first localize in Xenopus and zebrafish oocytes to the Balbiani body (Bb), a large membrane-less organelle conserved from insects to humans. The Bb is transient, disassembling and anchoring at one pole the axis-determining transcripts that establish the vegetal pole of the oocyte. Aggregation of the Bb depends on the Bucky ball (Buc) protein, an intrinsically disordered protein with a prion-like self-aggregation domain. In zebrafish buc null mutants, the Bb fails to form and oocytes lack polarity. Here, we report the newly established buc hypomorphic mutants that fail to form the Bb, but remarkably Buc protein and vegetal mRNAs localized normally at the vegetal cortex of the oocyte. Thus, these buc hypomorphic mutants displayed normal oocyte polarity, demonstrating that the Bb is not required to establish oocyte polarity.
HostingRepository	PRIDE
AnnounceDate	2026-09-08
AnnouncementXML	Submission_2026-09-08_07:48:14.386.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hossein Fazelinia
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: NEWT:7955;
ModificationList	monomethylated residue; phosphorylated residue
Instrument	timsTOF HT

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-31 20:26:14	ID requested
⏵ 1	2026-09-08 07:48:14	announced

Dataset with its publication pending

submitter keyword: buc protein, Balbiani body, Aggregation, Oocyte,Cell polarity

Mary C. Mullins, Ph.D.
contact affiliation	University of Pennsylvania Cell and Developmental Biology 1152 BRB II/III 421 Curie Boulevard Philadelphia, PA 19104-6058
contact email	mullins@pennmedicine.upenn.edu
lab head
Hossein Fazelinia
contact affiliation	Children's Hospital of Philadelphia
contact email	fazeliniah@email.chop.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076436

PRIDE project URI

• PRIDE
  1. PXD076436
     1. Label: PRIDE project
     2. Name: Oocyte polarity is established independently of the Balbiani body