PXD076414-1

PXD076414 is an original dataset announced via ProteomeXchange.

Title	Fungal extracellular vesicles are recoverable across variable ultracentrifugation speeds but display species-specific profiles of sedimentation
Description	Extracellular vesicles (EVs) are central components of fungal biology, yet their isolation commonly relies on ultracentrifugation schemes originally developed for mammalian systems. In Cryptococcus, most EVs carry the capsular polysaccharide glucuronoxylomannan (GXM), raising the possibility that vesicle density, and therefore sedimentation, could be influenced by polysaccharide content. Here, we systematically evaluated EV recovery across sequential ultracentrifugation speeds (20,000 × g to 100,000 × g) in Cryptococcus deuterogattii, Candida auris, and Candida parapsilosis. In C. deuterogattii, GXM was detected across all ultracentrifugation fractions, and each fraction efficiently transferred polysaccharide to acapsular cells, demonstrating that its presence is independent of fraction density and does not correlate with sedimentation behavior. In the three fungal species, transmission electron microscopy and nanoparticle tracking analysis confirmed that bona fide EVs are recoverable across all centrifugation speeds, with comparable size distributions. Qualitative and quantitative proteomic analyses revealed largely overlapping protein compositions among fractions within each species. However, proteomic profiles differed between species. In C. deuterogattii and C. auris, fractions displayed similar proteomic and predicted protein-protein interaction signatures across centrifugation speeds. In contrast, C. parapsilosis exhibited a clear partitioning pattern, with low-speed fractions (20,000 × g and 40,000 × g) clustering together and differing from higher-speed fractions (60,000–100,000 × g). These findings demonstrate that fungal EVs are recoverable across a broad range of ultracentrifugation speeds, but their sedimentation behavior is species-specific. Our study highlights the need for tailored EV isolation strategies and cautions against assuming methodological equivalence across fungal pathogens.
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:27:17.991.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marlon D M Santos
SpeciesList	scientific name: Cryptococcus neoformans RCT29; NCBI TaxID: NEWT:1160107;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-31 13:08:00	ID requested
⏵ 1	2026-06-28 16:27:18	announced

10.1007/s00232-026-00386-3;

Jozefowicz LJ, Bezerra BT, Veres E, Szilovics Z, Souza CM, Camillo-Andrade AC, Santos HGG, Carvalho PC, Santos MDM, Reis FCG, Gacser A, Rodrigues ML, Fungal Extracellular Vesicles are Recoverable Across Variable Ultracentrifugation Speeds but Display Species-specific Profiles of Sedimentation. J Membr Biol, 259(1):(2026) [pubmed]

submitter keyword: Fungal extracellular

vesicles

ultracentrifugation

profiles

Marcio L. Rodrigues
contact affiliation	Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil
contact email	marcio.rodrigues@fiocruz.br
lab head
Marlon D M Santos
contact affiliation	Computational Mass Spectrometry & Proteomics Group - Fiocruz
contact email	marlondms@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076414
     1. Label: PRIDE project
     2. Name: Fungal extracellular vesicles are recoverable across variable ultracentrifugation speeds but display species-specific profiles of sedimentation