PXD076406 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Lysine iminylation derived from ω-3 polyunsaturated fatty acids |
| Description | Protein post-translational modifications (PTMs) play a central role for regulating protein function and cellular processes, with many PTMs arising from reactions with electrophilic metabolites. Here we extend the known landscape of PTMs with the discovery of ‘lysine C3-iminylation’, the conjugation of protein lysine residues with propionaldehyde. To stabilize iminylation for mass spectrometric analyses and distinguish it from other isomeric PTMs, we developed a fixation and stable-isotope labeling approach based on parallel reduction of proteome with sodium borohydride and borodeuteride. Analyses of protein hydrolysates confirmed the presence of C3-iminylation in C. elegans and mouse. Additionally, proteomics results demonstrated specificity of this PTM in vitro and in vivo and revealed C3-iminylation in proteins related to critical metabolic pathways. Importantly, collective evidence from isotope tracing as well as genetic, dietary, and pharmacological manipulation experiments uncovered that C3-iminylation originates from cytochrome P450 (CYP)-mediated oxidation of omega-3 fatty acids. Correspondingly, C3-iminylation levels were elevated in C. elegans daf-2(e1370) mutants, an aging model, in which CYP activity is generally increased. These findings not only expand our understanding of the biochemical diversity of PTMs but also underscore the complex interplay between lipid metabolism and protein modifications, enabling further exploration of their biological and clinical implications. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-19 |
| AnnouncementXML | Submission_2026-08-19_08:33:41.784.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yu-Heng Hsieh |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Caenorhabditis elegans; NCBI TaxID: NEWT:6239; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-31 07:11:40 | ID requested | |
| ⏵ 1 | 2026-08-19 08:33:42 | announced | |
Publication List
Keyword List
| submitter keyword: proteomics, C. elegans, cytochrome P450 , omega-3 polyunsaturated fatty acid,post-translational modification |
Contact List
| Frank Schroeder |
|---|
| contact affiliation | Cornell University, Boyce Thompson Institiute |
| contact email | schroeder@cornell.edu |
| lab head | |
| Yu-Heng Hsieh |
|---|
| contact affiliation | Cornell University |
| contact email | yh998@cornell.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD076406
- Label: PRIDE project
- Name: Lysine iminylation derived from ω-3 polyunsaturated fatty acids
