PXD076375-1

PXD076375 is an original dataset announced via ProteomeXchange.

Title	Interactome of CD63 in WT and ATG16L1-KO cells
Description	We use an MS-based proteomic approach to demonstrate the physical interaction between the exosomal marker CD63 and mutant huntingtin, likely occurring upon transfer of the latter within the MVBs compartment, confirming how such pathway diversion can indeed regard autophagy substrate proteins (and other cytosolic cargoes) upon autophagy compromise or in response to other stimuli. In order to do so, we did perform the same experiments, namely transiently over-expressing GFP/CD63 and FLAG/mutant huntingtin in WT and ATG16L1-KO and performed GFP-trap experiments starting from four different samples (per genotype). Immuno-isolated samples were then subjected to mass spectrometry analysis. Interestingly, upon comparative analysis and gene identification, around 5700 proteins were detected. Out of these, we could report 126 proteins interacting differentially with CD63 in autophagy-dependent manner. Finally, the MS-approach was also instrumental in confirming, the lack of any significant change in the interaction between CD63 and mutant huntingtin as a function of the different autophagy background.
HostingRepository	PRIDE
AnnounceDate	2026-05-13
AnnouncementXML	Submission_2026-05-13_08:49:04.769.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michele Costanzo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-31 06:33:50	ID requested
⏵ 1	2026-05-13 08:49:05	announced

Dataset with its publication pending

submitter keyword: None

Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology - University of Naples Federico II
contact email	michele.costanzo@unina.it
lab head
Michele Costanzo
contact affiliation	Department of Molecular Medicine and Medical Biotechnology - University of Naples Federico II
contact email	michele.costanzo@unina.it
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD076375

PRIDE project URI

• PRIDE
  1. PXD076375
     1. Label: PRIDE project
     2. Name: Interactome of CD63 in WT and ATG16L1-KO cells