PXD076268-1

PXD076268 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation of rhizobial effector NopZ by soybean NORK promotes association with NENA and enhances nodulation
Description	Legume-rhizobial symbiosis, involving the intracellular accommodation of rhizobia within host cells for nitrogen fixation, represents a unique model among plant-microbe interactions. While this symbiosis requires sophisticated regulatory networks, direct host control over symbiont proteins remains largely elusive. Here, we demonstrate that soybean Nodulation Receptor Kinase (GmNORK) interacts with and directly phosphorylates the Sinorhizobium fredii HH103 effector NopZ. This host-mediated phosphorylation promotes NopZ nuclear/perinuclear enrichment and is associated with enhanced NopZ interaction with the nucleoporin GmNENA at the nuclear envelope/nuclear pore complex, establishing a key signaling step that links membrane-proximal symbiotic signaling to the nuclear pore. Expression of phosphomimetic NopZ variants enhances soybean nodulation, whereas GmNENA-silencing in soybean roots reduces nodule numbers. Our findings reveal a previously unknown model for plant-microbe interactions, where the direct phosphorylation of a bacterial effector by a host receptor kinase provides an essential regulatory mechanism to direct effector localization and promote the symbiotic program.
HostingRepository	PRIDE
AnnounceDate	2026-04-10
AnnouncementXML	Submission_2026-04-10_03:00:29.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yutao Lei
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 14:07:40	ID requested
⏵ 1	2026-04-10 03:00:30	announced

Dataset with its publication pending

submitter keyword: soybean;phosphorylation;effector;NPC

Yangrong Cao
contact affiliation	National Key Lab of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University
contact email	yrcao@mail.hzau.edu.cn
lab head
Yutao Lei
contact affiliation	Huazhong agricaltural university
contact email	541198867@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076268
     1. Label: PRIDE project
     2. Name: Phosphorylation of rhizobial effector NopZ by soybean NORK promotes association with NENA and enhances nodulation