PXD076256-1

PXD076256 is an original dataset announced via ProteomeXchange.

Title	Conformational changes of shapeshifting protein G’e and immunoglobulin IgG probed by nanoESI ion mobility mass spectrometry.
Description	Integrative structural biology approaches make use of artificial intelligence-assisted modelling on one side and of experimental analyses on the other for in-depth characterization of higher order protein structures as well as physiological structural changes. Calculations of CCS values are based on either three-dimensional (3D) protein structures or 3D structure models, resembling protein structures in condensed phase. Measuring collision cross sections (CCS) of proteins by nano-electrospray mass spectrometry is rapidly performed with consuming only little material and provides CCS of protein ions in the gas phase. Interestingly, calculated and experimentally determined CCS match well as long as 3D structures show that peptides, proteins, and complexes are compactly folded. Hence, gas phase CCS values provide useful information about higher order solution structures. Deviations between gas phase CCS values and those from compact 3D solution structures are found as well and lend evidence that in solution such shapeshifting proteins adopt more than one conformation.
HostingRepository	PRIDE
AnnounceDate	2026-09-01
AnnouncementXML	Submission_2026-08-31_22:11:18.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Kreutzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460; scientific name: Gallus gallus (Chicken); NCBI TaxID: NEWT:9031; scientific name: Streptococcus sp. 'group G'; NCBI TaxID: NEWT:1320; scientific name: Bos taurus (Bovine); NCBI TaxID: NEWT:9913; scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: NEWT:9825;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 10:31:46	ID requested
⏵ 1	2026-08-31 22:11:19	announced

10.3390/IJMS27177662;

submitter keyword: Offline ESI-MS, IMS-MS

Prof. Dr. Michael O. Glocker
contact affiliation	Proteome Center Rostock, University of Rostock, Germany
contact email	michael.glocker@uni-rostock.de
lab head
Michael Kreutzer
contact affiliation	University Medical Center Rostock
contact email	michael.kreutzer@med.uni-rostock.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD076256

PRIDE project URI

• PRIDE
  1. PXD076256
     1. Label: PRIDE project
     2. Name: Conformational changes of shapeshifting protein G’e and immunoglobulin IgG probed by nanoESI ion mobility mass spectrometry.