PXD076230-1

PXD076230 is an original dataset announced via ProteomeXchange.

Title	Targeting IRF1-TRIM21 axis Enhances Anti-Tumor Immunity by Promoting ubiquitin-mediated degradation of FGL1 in non-small cell lung cancer
Description	Immunotherapy has emerged as a first-line treatment for patients with advanced non-small cell lung cancer (NSCLC), but over half of patients fail to benefit, largely due to tumor heterogeneity and a complex tumor microenvironment. Here, we demonstrate that fibrinogen-like protein 1 (FGL1), a recently identified immune checkpoint ligand, is targeted by tripartite motif-containing protein 21 (TRIM21) for proteasomal degradation, thereby enhancing the anti-tumor activity of cytotoxic T lymphocytes (CTLs). We further show that interferon regulatory factor 1 (IRF1) regulates TRIM21 transcription. Artemisinin upregulates the IRF1-TRIM21 axis, promoting FGL1 degradation. In high FGL1 expressed tumors, artemisinin in combination with anti-programmed cell death protein 1 (anti-PD-1) therapy enhances immunotherapeutic efficacy. Clinically, an elevated FGL1/TRIM21 protein ratio is associated with poor prognosis in NSCLC. Collectively, these findings elucidate a post-translational regulatory mechanism of FGL1 in tumor progression and support the development of a rational combination immunotherapy strategy for NSCLC treatment.
HostingRepository	iProX
AnnounceDate	2026-03-27
AnnouncementXML	Submission_2026-09-09_23:56:46.591.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuchen Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Ultra

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-27 01:34:41	ID requested
⏵ 1	2026-09-09 23:56:47	announced

Zhang Y, Zhou P, Guo Y, Zhang H, Zhao G, Yin J, Ge D, Liu R, Gu J, Zhang C, Targeting IRF1-TRIM21 axis enhances anti-tumor immunity by promoting ubiquitin-mediated degradation of FGL1 in non-small cell lung cancer. Commun Biol, 9(1):(2026) [pubmed]

submitter keyword: FGL1, TRIM21, ubiquitination, IRF1, anti-tumor immunity

Chunyi Zhang
contact affiliation	Fudan University
contact email	cyzhang@fudan.edu.cn
lab head
Yuchen Zhang
contact affiliation	Fudan University
contact email	zhangyc7097@163.com
dataset submitter

iProX dataset URI