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PXD076164-1
PXD076164 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis |
| Description | Genetic mapping is a powerful tool for eukaryotic genetics that has only been applied to bacteria in limited circumstances. Quantitative trait locus (QTL) mapping generally relies on sexual recombination to break linkages between genes, yet bacteria rarely undergo sufficient homologous recombination to generate suitable mapping populations. In this work, we used iterative biparental genome shuffling by protoplast fusion in Bacillus subtilis to generate a population of bacteria with substantial random recombination throughout their genomes. Individual shuffled progeny were arrayed in well plates, resequenced, and characterized for a range of complex phenotypes including spore germination and swarming motility. Genetic mapping of the resulting phenotypes identified high-confidence QTLs of moderate size (~15 kb), and these associations were validated through targeted genetic swaps. This B. subtilis QTL population can easily be used to map additional phenotypes and the general approach for QTL mapping is applicable in a wide range of bacteria. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-26 |
| AnnouncementXML | Submission_2026-03-26_04:15:45.879.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dana Carper |
| SpeciesList | scientific name: Bacillus subtilis; NCBI TaxID: 1423; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-25 14:15:28 | ID requested | |
| ⏵ 1 | 2026-03-26 04:15:46 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Bacillus subtilis, QTL mapping, protoplast fusion, DatasetType:Proteomics |
Contact List
| Richard J. Giannone | |
|---|---|
| contact affiliation | Oak Ridge National Laboratory |
| contact email | giannonerj@ornl.gov |
| lab head | |
| Dana Carper | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | carperdl@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101244/ |
