PXD076163-1

PXD076163 is an original dataset announced via ProteomeXchange.

Title	Autophagy-driven Presynaptic Reorganization as a Molecular Signature of Brain Resilience
Description	Neural circuits must maintain functional stability while remaining adaptable to changing demands and stress. This balance is thought to rely on plasticity programs that integrate molecular and activity-dependent signals to structurally and functionally reconfigure synapses; however, the underlying mechanisms remain poorly understood. Here, we demonstrate that targeted impairment of autophagy in the Drosophila mushroom body (MB) induces brain-wide post-transcriptional remodeling at presynaptic active zones (AZ). This remodeling is characterized by the selective upregulation of AZ scaffold proteins, reduced levels of calcium channel subunits, and increased abundance of Shaker-type potassium channels. Functionally, this AZ remodeling program enhances resilience to local autophagy disruption, as evidenced by increased sleep and extended lifespan. Moreover, MB-specific autophagy impairment leads to a non-cell-autonomous accumulation of autophagic substrates across the brain, indicating systemic propagation of proteostatic stress. Together, our findings identify MB autophagy as a key regulator of synaptic architecture and sleep-associated resilience, and establish a genetically tractable model for how localized stress signals can drive brain-wide adaptive responses through synaptic reprogramming.
HostingRepository	PRIDE
AnnounceDate	2026-07-06
AnnouncementXML	Submission_2026-07-06_03:27:47.544.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pin-Lian Jiang
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	ubiquitination signature dipeptidyl lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	quadrupole orbitrap astral instrument

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-25 13:47:06	ID requested
⏵ 1	2026-07-06 03:27:48	announced

10.1038/S44318-026-00847-4;

submitter keyword: ubiquitinome, synapse, brain, Digly, Drome, mushroom body

Fan Liu
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie
contact email	fliu@fmp-berlin.de
lab head
Pin-Lian Jiang
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie
contact email	jiang@fmp-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD076163
     1. Label: PRIDE project
     2. Name: Autophagy-driven Presynaptic Reorganization as a Molecular Signature of Brain Resilience