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PXD076033-1
PXD076033 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Stage- and stress-specific phosphorylation of ribosomal protein Rps5 (uS7) by Smk1 and Kns1 kinases in Saccharomyces cerevisiae. |
| Description | In the yeast Saccharomyces cerevisiae, nutrient deprivation triggers cell-type-specific responses: haploid cells exit the mitotic cycle and enter stationary phase, while diploid cells undergo meiosis and form spores. The meiosis-specific mitogen-activated protein kinase (MAPK) Smk1 regulates spore formation. The ubiquitously expressed Cdc2-like kinase (CLK) Kns1 downregulates ribosomal RNA and tRNA synthesis in mitotically dividing cells under nutrient-deprived conditions. Here, we show that Smk1 phosphorylates ribosomal protein Rps5 (uS7) on threonine residues 21 and 27 during the early stages of spore formation. As spore formation progresses, Kns1 is required to further increase the phosphorylation of these residues. Kns1-dependent phosphorylation of Rps5 is also observed in nutrient-deprived or rapamycin-treated haploid cells. Upon exposure to nutrient-rich media and spore germination, ribosomal subunits containing unphosphorylated Rps5 are incorporated into translationally active polysomes, while phosphorylated Rps5 is retained in inactive 80S ribosomes. Taken together, our data support a model in which Smk1 and Kns1 cooperate across the yeast life cycle to promote Rps5 phosphorylation and thereby bias a subset of ribosomes toward translational quiescence. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-25 |
| AnnouncementXML | Submission_2026-03-25_16:40:54.688.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Hsin-Yao Tang |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | Oxidation; Carbamidomethyl |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-23 12:14:16 | ID requested | |
| ⏵ 1 | 2026-03-25 16:40:55 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Smk1, Kns1, Rps5, meiosis, sporulation, TORC, small ribosomal subunit, translation, DatasetType:Proteomics |
Contact List
| Edward Winter | |
|---|---|
| contact affiliation | Thomas Jefferson University |
| contact email | edward.winter@jefferson.edu |
| lab head | |
| Hsin-Yao Tang | |
| contact affiliation | The Wistar Institute |
| contact email | tangh@wistar.org |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101215/ |
