PXD076025-1

PXD076025 is an original dataset announced via ProteomeXchange.

Title	Differential Proteomic Landscape of Plasma Neuron-Derived Extracellular Vesicles in Parkinson’s Disease with and without RBD
Description	Parkinson’s disease (PD) is increasingly recognized as a heterogeneous disorder, with rapid eye movement sleep behavior disorder (RBD) serving as a key prodromal marker for a distinct, aggressive subtype. This study investigates the plasma neuron-derived extracellular vesicle (EV) proteome to delineate molecular differences between PD patients with RBD (PD-RBD) and those without (PD-noRBD). Using L1CAM-based immunoprecipitation and Data-independent acquisition mass spectrometry (DIA-MS) proteomics, we identified 1,354 proteins across 28 subjects. Comparative analysis revealed 239 differentially expressed proteins (DEPs) between PD-RBD and PD-noRBD. Functional enrichment and PPI network analyses uncovered divergent pathobiological signatures: PD-noRBD was dominated by dysregulated lipid metabolism (APOE, CETP, PLTP) and systemic inflammation, while PD-RBD exhibited pronounced extracellular matrix remodeling (COL3A1, MMP7, VCAN) and complement activation. Correlations with clinical features and brain iron deposition (via quantitative susceptibility mapping, QSM) further linked specific DEPs to motor severity, disease duration, and autonomic symptoms. Notably, α-synuclein (SNCA) levels were significantly higher in PD-RBD. A series of biomarker candidates in differentiating subtypes were identified. These findings provide compelling evidence that PD-RBD and PD-noRBD represent distinct molecular entities, driven by divergent pathogenic mechanisms involving metabolic-inflammation versus structural-extracellular matrix (ECM) pathways. This proteomic map offers a valuable resource for developing subtype-specific diagnostic tools and targeted therapeutic strategies, advancing the field toward precision medicine for Parkinson’s disease.
HostingRepository	PRIDE
AnnounceDate	2026-08-27
AnnouncementXML	Submission_2026-08-27_00:38:04.662.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhang Zhou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-23 07:56:15	ID requested
⏵ 1	2026-08-27 00:38:05	announced

10.2147/IJN.S616053;

submitter keyword: Neuron-derived extracellular vesicle, α-Synuclein, Lipid metabolism, hetrogenity, L1CAM, Rapid eye movement sleep behavior disorder (RBD), Proteomics, Extracellular matrix remodeling, Subtype stratification, precision medicine,Parkinson’s disease, Biomarkers

Yuhang ZHOU
contact affiliation	1. Shengli Clinical Medical College of Fujian Medical University, Fujian Provincial Hospital, Fuzhou University Affiliated Provincial Hospital, Department of Neurology, Fuzhou 350108, China 2.School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China
contact email	yuhangzhou1@link.cuhk.edu.cn
lab head
Yuhang Zhou
contact affiliation	The Chinese University of Hong Kong, Shenzhen
contact email	yuhangzhou1@link.cuhk.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD076025

PRIDE project URI

• PRIDE
  1. PXD076025
     1. Label: PRIDE project
     2. Name: Differential Proteomic Landscape of Plasma Neuron-Derived Extracellular Vesicles in Parkinson’s Disease with and without RBD