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PXD075951-1

PXD075951 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSucrose-Activated TOR and phyA Signaling Alleviates Shade-Mediated Inhibition of Leaf Development in Arabidopsis
DescriptionTo investigate how the sucrose-TOR signaling pathway regulates phyA, we conducted LC-MS/MS analysis to identify phosphorylation sites on phyA. In vivo, the phyA protein was immunoprecipitated from 35S::PHYA-YFP/phyA-211 seedlings treated with shade and 30 mM sucrose for 6 hours. In vitro, we used recombinant phyA protein purified from insect cells as the substrate and endogenous TOR complex immunoprecipitated from the 35S::LST8-1-FLAG transgenic line as the kinase source. Phosphopeptide enrichment and LC–MS/MS analysis of the in vitro reaction were performed.
HostingRepositoryPRIDE
AnnounceDate2026-03-21
AnnouncementXMLSubmission_2026-03-20_21:29:04.636.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJiayu Wang
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationListphosphorylated residue
Instrumentultraflex; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02026-03-20 10:52:51ID requested
12026-03-20 21:29:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Shade avoidance
sucrose
phyA
TOR
leaf development
Contact List
Lin Li
contact affiliationUniversity of Fudan
contact emaillinli@fudan.edu.cn
lab head
Jiayu Wang
contact affiliationUniversity of Fudan
contact email24110700078@m.fudan.edu.cn
dataset submitter
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Dataset FTP location
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