PXD075899-1

PXD075899 is an original dataset announced via ProteomeXchange.

Title	Affinity purification mass spectrometry
Description	To identify potential ligands of Siglec-G in the GBM microenvironment, a Siglec-G-Fc pull-down assay followed by mass spectrometry analysis was performed. Briefly, recombinant Siglec-G-Fc (Novoprotein, Cat. No.:C05K) fusion protein containing the extracellular domain of Siglec-G fused to the human IgG1 Fc region was incubated with either glioma tumor tissue lysates derived from tumor-bearing mice or GL261 glioma cell lysates. Human IgG1-Fc (Novoprotein, Cat. No.:C30D) was used as a negative control. The mixtures were incubated at 4 °C with rotation to allow binding between Siglec-G and potential ligands. Immune complexes were captured using Protein A/G agarose beads and extensively washed with lysis buffer to remove non-specifically bound proteins. Bound proteins were subsequently eluted and separated by SDS-PAGE. Gel bands were excised and subjected to in-gel trypsin digestion. The resulting peptides were analyzed by LC-MS. Candidate ligands were identified by comparing proteins enriched in the Siglec-G-Fc samples relative to the IgG1-Fc control. Proteins were further filtered based on peptide abundance and cell surface localization to prioritize potential Siglec-G-binding ligands.
HostingRepository	iProX
AnnounceDate	2026-03-19
AnnouncementXML	Submission_2026-03-19_20:27:56.224.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yanan Wang
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-19 20:27:40	ID requested
⏵ 1	2026-03-19 20:27:56	announced

Dataset with its publication pending

submitter keyword: Affinity purification

Yanan Wang
contact affiliation	School of Immunology, Nanjing Medical University
contact email	1655563426@qq.com
lab head
Yanan Wang
contact affiliation	School of Immunology, Nanjing Medical University
contact email	1655563426@qq.com
dataset submitter

iProX dataset URI