PXD075836 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mammalian MemPrep establishes the lipid composition ER membranes in HEK293T cells |
| Description | Mammalian MemPrep is an approach that enables the isolation of ER membranes from HEK293T cells at a purity suitable for quantitative proteomics and lipidomics. Using cleavable, epitope‑tagged variants of SEC61β and REEP5, which preferentially localize to ER sheets and tubules, respectively, we isolate membrane vesicles corresponding to the two major ER subdomains. The proteomic analysis confirms the high purity of our preparation and the expected distribution of protein machineries: sheet‑associated factors, including translocon components and ribosomal proteins, are enriched in SEC61β isolates, whereas curvature‑stabilizing proteins are enriched in REEP5 isolates. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-03 |
| AnnouncementXML | Submission_2026-09-02_23:03:40.375.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Frank Stein |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-18 22:23:37 | ID requested | |
| ⏵ 1 | 2026-09-02 23:03:41 | announced | |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: ER membrane composition, immunoisolation, mammalian cells,MemPrep, differential centrifugation |
Contact List
| Robert Ernst |
|---|
| contact affiliation | Universität des Saarlandes |
| contact email | robert.ernst@uks.eu |
| lab head | |
| Frank Stein |
|---|
| contact affiliation | EMBL |
| contact email | frank.stein@embl.de |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075836 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075836
- Label: PRIDE project
- Name: Mammalian MemPrep establishes the lipid composition ER membranes in HEK293T cells
