PXD075811 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Evaluation of protein artifacts in tissue cryopreservation |
| Description | This dataset presents proteomic analyses validating the spatial proteomics technology, NicheProt. NicheProt is a 3D optical microscopy–guided, photobleaching-mediated cell barcoding approach for isolating intact cell types from defined microanatomical niches. Here, we evaluate whether tissue cryopreservation introduces protein artifacts by comparing proteomic profiles of mouse spleens processed immediately after fixation with those cryopreserved in 100% D-fructose at −80 °C prior to extraction. Our results demonstrate that D-fructose–based cryopreservation preserves protein integrity without introducing significant artifacts. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-19 |
| AnnouncementXML | Submission_2026-03-19_13:00:08.345.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD075811 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Steve Seung-Young Lee |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-18 16:18:01 | ID requested | |
| ⏵ 1 | 2026-03-19 13:00:08 | announced | |
Publication List
Keyword List
Contact List
| Steve Seung-Young |
|---|
| contact affiliation | Department of Pharmaceutical Sciences, University of Illinois College of Pharmacy,Chicago,IL,USA Richard and Loan Hill Department of Biomedical Engineering, University of Illinois College of Engineering,Chicago, IL,USA University of Illinois Cancer Center,Chicago, IL,USA |
| contact email | ssylee@uic.edu |
| lab head | |
| Steve Seung-Young Lee |
|---|
| contact affiliation | University of Illinois Chicago |
| contact email | ssylee@uic.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD075811 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075811
- Label: PRIDE project
- Name: Evaluation of protein artifacts in tissue cryopreservation
