PXD075809-1

PXD075809 is an original dataset announced via ProteomeXchange.

Title	Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities
Description	Proteolysis is a universal process, as proteases play a pivotal role in modulating numerous signaling pathways. By hydrolyzing peptide bonds within their target proteins, proteases control their fate and function. Understanding the temporal and spatial dynamics of proteolytic events, including the proteases that execute them, is crucial for elucidating their particular roles across diverse biological processes. In this study, we developed and characterized a set of genetically encoded Förster resonance energy transfer (FRET)-based reporters for the detection of various proteolytic activities in plants. Our sensors reliably reported the activity of specific proteases, exhibiting performance comparable to established detection systems. In addition, we engineered variants capable of detecting the spatial dynamics of metacaspase-triggered proteolysis after wounding and during programmed cell death in roots. The implementation of these tools in plant research opens new opportunities to explore proteolytic mechanisms with enhanced precision. We demonstrate the feasibility of the FRET-based sensors to detect various activities in vivo with high spatiotemporal resolution. Overall, these biosensors constitute a versatile toolbox for probing protease function within its native cellular context, paving the way for deeper insights into plant biology and signaling. This dataset includes a targeted shotgun proteomics experiment designed to identify the specific endoproteolytic cleavage products generated from the FRET(VRPR) biosensor.
HostingRepository	PRIDE
AnnounceDate	2026-07-04
AnnouncementXML	Submission_2026-07-04_11:34:55.814.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jingjing Huang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: NEWT:3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-18 13:29:06	ID requested
⏵ 1	2026-07-04 11:34:56	announced

10.1093/PLCELL/KOAG162;

submitter keyword: None

Frank Van Breusegem
contact affiliation	1 Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium 2 Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact email	frank.vanbreusegem@psb.vib-ugent.be
lab head
Jingjing Huang
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, 9052 Ghent, Belgium
contact email	jingjing.huang@ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/07/PXD075809

PRIDE project URI

• PRIDE
  1. PXD075809
     1. Label: PRIDE project
     2. Name: Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities