PXD075760-1

PXD075760 is an original dataset announced via ProteomeXchange.

Title	An Lsr2-like xenogeneic silencer confers immunity against AT-rich bacteriophage infection
Description	Lsr2-like xenogeneic silencers are widespread nucleoid-associated proteins in Actinomycetota and are encoded by diverse bacteriophages. However, their potential roles during bacteriophage infection, one of the most direct encounters with foreign DNA, remain unexplored. In Corynebacterium glutamicum, the Lsr2-like protein CgpS is encoded within the mobile genetic element CGP3 and is known to silence transcription of foreign DNA within this region. Here we show that CgpS also restricts infection by the AT-rich bacteriophage Jean Grey. Upon phage DNA injection, CgpS binds phage-derived AT-rich sequences and represses early phage gene transcription. This transcriptional bottleneck prevents efficient phage genome replication and delays progression of the viral program. The resulting temporal window enables activation of host stress and defense pathways, including LexA-regulated SOS genes and additional antiviral systems. Our findings reveal a novel antiviral function for an Lsr2-like silencer and suggest that xenogeneic silencers can act as regulatory barriers that modulate the outcome of phage infection in Actinomycetota.
HostingRepository	PRIDE
AnnounceDate	2026-03-21
AnnouncementXML	Submission_2026-03-21_04:24:32.558.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Konstantinos Kalogeropoulos
SpeciesList	scientific name: Corynebacterium glutamicum MB001; NCBI TaxID: NEWT:1310161;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-17 08:48:01	ID requested
⏵ 1	2026-03-21 04:24:33	announced

Dataset with its publication pending

submitter keyword: proteomics, DIA,Corynebacterium glutamicum, bacteriophage, infection

Konstantinos Kalogeropoulos
contact affiliation	Technical University of Denmark
contact email	konka@dtu.dk
lab head
Konstantinos Kalogeropoulos
contact affiliation	Technical University of Denmark
contact email	konskalogero@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075760
     1. Label: PRIDE project
     2. Name: An Lsr2-like xenogeneic silencer confers immunity against AT-rich bacteriophage infection