PXD075718 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | NicheProt: Cell-type-resolved proteomics of mouse spleen tissue compartments |
| Description | This dataset contains proteomic analyses that validate the spatial proteomic technology, NicheProt, and its application to investigate dendritic cell phenotypes in inflammed mouse spleens. NicheProt is a 3D optical microscopy-guided, photobleaching-mediated cell barcoding approach to isolate intact cell types from specific microanatomical niches. Integrating with sequential bottom-up LC-MS/MS analysis we identified two distinct CD11c⁺ dendritic cell phenotypes defined by their spatial distribution. These compartment-specific populations displayed differential expression of 54 proteins. This approach enables cell-type and microregion-resolved proteomic insights, revealing previously unrecognized cell subtypes and their roles within distinct tissue compartments. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-03-19 |
| AnnouncementXML | Submission_2026-03-19_13:00:24.900.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD075718 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Steve Seung-Young Lee |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-16 21:57:04 | ID requested | |
| ⏵ 1 | 2026-03-19 13:00:25 | announced | |
Publication List
Keyword List
| submitter keyword: microscopy,dendritic cells, inflammation, cell barcoding, spatial proteomics |
Contact List
| Steve Seung-Young Lee |
|---|
| contact affiliation | Department of Pharmaceutical Sciences, University of Illinois College of Pharmacy,Chicago,IL,USA Richard and Loan Hill Department of Biomedical Engineering, University of Illinois College of Engineering,Chicago, IL,USA University of Illinois Cancer Center,Chicago, IL,USA |
| contact email | ssylee@uic.edu |
| lab head | |
| Steve Seung-Young Lee |
|---|
| contact affiliation | University of Illinois Chicago |
| contact email | ssylee@uic.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD075718 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075718
- Label: PRIDE project
- Name: NicheProt: Cell-type-resolved proteomics of mouse spleen tissue compartments
