PXD075695 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Biofilm-associated extracellular material in Shewanella oneidensis MR-1 |
| Description | Biofilms formed by Shewanella oneidensis MR-1 are crucial for metal reduction, underpinning bioremediation and bioenergy applications, yet detailed structural insights into biofilm components remain limited. Here we show that MR-1 biofilms contain abundant filamentous networks, membrane vesicles, and distinct square-shaped aggregates, as revealed by cryo-electron tomography and cryo-electron microscopy. We further determine near-atomic resolution structures of three filament types: bacterial flagella and two distinct pili, PilA and MshA. Notably, we present high-resolution structure of an MshA pilus and a PilA pilus structure from Shewanella. Structural analyses show that MshA pili exhibit a balanced surface charge distribution and extensive solvent-accessible surface area, facilitating essential interactions within the biofilm matrix. Additionally, oxygen limitation markedly increases the abundance of extracellular filaments and protein aggregates, indicating adaptive responses to environmental stress. Our findings elucidate the fundamental architecture and roles of biofilm extracellular components and provide a structural foundation for engineering enhanced Shewanella strains. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-07_04:20:47.227.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuxuan Yao |
| SpeciesList | scientific name: Shewanella oneidensis (strain MR-1); NCBI TaxID: NEWT:211586; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-16 11:36:25 | ID requested | |
| ⏵ 1 | 2026-09-07 04:20:48 | announced | |
Publication List
| Li D, Dong H, Zhang G, Li Y, Liu X, Chen Y, Xiao L, Wang X, Li D, Song H, Zhong C, Cao Q, Dai B, Liu C, Ultrastructural and atomic characterization of biofilm-associated extracellular filaments in Shewanella oneidensis MR-1. Nat Commun, 17(1):(2026) [pubmed] |
| 10.1038/s41467-026-72442-4; |
Keyword List
| submitter keyword: Biofilm,Shewanella oneidensis |
Contact List
| Cong Liu |
|---|
| contact affiliation | Interdisciplinary Research Center on Biology and Chemistry, State Key Laboratory of Chemical Biology, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China |
| contact email | ldn998899@163.com |
| lab head | |
| Yuxuan Yao |
|---|
| contact affiliation | Shanghai Jiao Tong University |
| contact email | yaoyuxuan@sjtu.edu.cn |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075695
- Label: PRIDE project
- Name: Biofilm-associated extracellular material in Shewanella oneidensis MR-1
