PXD075694 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Prospective ICH Q2(R2)-aligned validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics |
| Description | This dataset contains raw and processed LC–MS/MS data generated for a prospective validation of label-free untargeted proteomics for host cell protein (HCP) quantification in biotherapeutic matrices. A stable isotope-labeled CHO whole-proteome standard was spiked into the NIST monoclonal antibody reference material (RM 8671) across seven concentration levels (20–80 ng total HCP per injection). Samples were analyzed using an Evosep One nanoLC system coupled to a Bruker timsTOF Pro operating in ddaPASEF mode. The study comprised four independent analytical assays totaling 198 injections and was designed to evaluate analytical performance characteristics under an ICH Q2(R2) total-error validation framework. The dataset supports evaluation of identification error control, quantitative trueness, precision, intermediate precision, and total error across the validated range. Raw Bruker timsTOF acquisition files and identification results generated with SpectroMine are provided together with the FASTA database and analysis parameters to ensure full reproducibility. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-08 |
| AnnouncementXML | Submission_2026-06-07_16:09:07.796.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Somar khalil |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | maXis |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-16 11:33:58 | ID requested | |
| ⏵ 1 | 2026-06-07 16:09:08 | announced | |
Publication List
| 10.3390/proteomes14020021; |
| Khalil S, Dierick JF, Bourguignon P, Plisnier M, Prospective ICH Q2(R2)-Aligned Total-Error Validation of Label-Free Untargeted Proteomics for Host Cell Protein Quantification in Biotherapeutics. Proteomes, 14(2):(2026) [pubmed] |
Keyword List
| submitter keyword: Host cell proteins, HCP quantification, biotherapeutics, timsTOF, analytical validation, untargeted proteomics, ddaPASEF, ICH Q2(R2), LC–MS/MS |
Contact List
| Somar Khalil |
|---|
| contact affiliation | Technical Research & Development, GSK |
| contact email | somar.x.khalil@gsk.com |
| lab head | |
| Somar khalil |
|---|
| contact affiliation | GSK |
| contact email | somar.x.khalil@gsk.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075694
- Label: PRIDE project
- Name: Prospective ICH Q2(R2)-aligned validation of label-free untargeted proteomics for host cell protein quantification in biotherapeutics
