PXD075602 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Probing molecular diversity of brain cells with chemically modified aptamers |
| Description | We developed a volumetric correlated light and electron microscopy (CLEM) approach that uses slow off-rate modified aptamers (SOMAmers) for fluorescence labeling of cells in ultrastructurally reconstructable electron micrographs. The small size of aptamers enables intracellular labeling in fixed brain tissue without detergent permeabilization, thereby preserving tissue ultrastructure and allowing high-resolution electron microscopy imaging and accurate cellular segmentation. This method enables labeling of a wide range of molecular biomarkers, providing a powerful strategy to integrate molecular information with volumetric electron microscopy datasets. As a result, it facilitates molecularly annotated ultrastructural studies of complex tissues, including investigations of neural circuits. To ensure that the aptamers used in this method bind specifically to their intended protein targets, we validated target binding using pull-down mass spectrometry. These results were further confirmed through complementary approaches including genetic correlation analyses, proximity extension assays, and comparisons with commercial antibody labeling. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-17 |
| AnnouncementXML | Submission_2026-04-17_07:55:28.234.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xiaotang Lu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q-Tof Ultima |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-13 02:28:10 | ID requested | |
| ⏵ 1 | 2026-04-17 07:55:28 | announced | |
Publication List
Keyword List
| submitter keyword: neuroscience,pulldown MS |
Contact List
| Xiaotang Lu |
|---|
| contact affiliation | University of Illinois Urbana-Champaign |
| contact email | xiaotang@illinois.edu |
| lab head | |
| Xiaotang Lu |
|---|
| contact affiliation | University of Illinois Urbana-Champaign |
| contact email | xiaotang@illinois.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075602 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075602
- Label: PRIDE project
- Name: Probing molecular diversity of brain cells with chemically modified aptamers
