PXD075589 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic stable isotope probing (proteomic SIP) using 13C‑E. coli spike‑ins analyzed by DDA LC–MS/MS |
| Description | This project applies proteomic stable isotope probing (proteomic SIP) to evaluate detection and quantification of 13C‑labeled E. coli peptides within a complex mouse fecal microbiome background. Proteins were extracted, digested, and quantified before preparing spike‑in mixtures. Mouse fecal peptides (2 µg) were combined with E. coli peptides at 10:1 or 100:1 ratios using defined 13C labeling levels (2%, 5%, 50%), each in triplicate. Peptides were analyzed by nanoLC–MS/MS on an XSelect CSH C18 column coupled to an Orbitrap Fusion Tribrid mass spectrometer in data‑dependent acquisition mode. The dataset enables controlled evaluation of isotopic incorporation, quantitative behavior, and sensitivity of proteomic SIP in microbiome samples. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-30 |
| AnnouncementXML | Submission_2026-08-29_19:44:06.212.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yi Xiong |
| SpeciesList | scientific name: gut metagenome; NCBI TaxID: NEWT:749906; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-12 22:50:03 | ID requested | |
| ⏵ 1 | 2026-08-29 19:44:06 | announced | |
Publication List
Keyword List
| submitter keyword: proteomic SIP |
| stable isotope probing |
| metaproteomics |
| 13C labeling |
| E. coli spike‑in |
| mouse fecal microbiome |
| DDA |
| Orbitrap Fusion |
Contact List
| Chongle Pan |
|---|
| contact affiliation | School of Computer Science, School of Biomedical Engineering, the University of Oklahoma |
| contact email | cpan@ou.edu |
| lab head | |
| Yi Xiong |
|---|
| contact affiliation | The School of Biological Sciences, The University of Oklahoma |
| contact email | yixiong@ou.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD075589 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075589
- Label: PRIDE project
- Name: Proteomic stable isotope probing (proteomic SIP) using 13C‑E. coli spike‑ins analyzed by DDA LC–MS/MS
