PXD075581-1

PXD075581 is an original dataset announced via ProteomeXchange.

Title	Highlights of Tuft Cells in Mouse and Human Salivary Glands
Description	Tuft cells (TCs) are rare chemosensory epithelial cells that regulate mucosal homeostasis in several organs, but their role in salivary glands (SGs) remains unclear. This study investigated TC features in both mouse and human SGs. In mouse samples, transmission electron microscopy (TEM), histological analysis, bulk RNA-sequencing and proteomic analyses were performed on submandibular glands (SMGs) from female and male C57BL/6J (wild-type) and C57BL/6J-Pou2f3-/- mice, which lack TCs. Moreover, human minor SG biopsies were evaluated by immunostaining and Poisson regression to identify clinical, serological, and histopathological features correlated with TC abundance. TEM analysis confirmed conserved TC ultrastructure—apical microvilli, a tubulovesicular system, and cytospinules—in both female and male mouse SMGs. Moreover, TC loss was associated with sex-specific transcriptional remodeling (1742 differentially expressed genes in females versus 175 in males), morphological alterations and sex-specific saliva proteomic remodeling. In human minor SG biopsies, TC counts were correlated with salivary flow, serological and histopathology features. In summary, these findings suggest that TCs are regulators of epithelial integrity and potential biomarkers of SG dysfunction
HostingRepository	PRIDE
AnnounceDate	2026-04-27
AnnouncementXML	Submission_2026-04-26_16:11:06.866.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chao Xue
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-12 16:48:15	ID requested
⏵ 1	2026-04-26 16:11:07	announced

Rusiniak ME, Shimagami L, Drumond VZ, Souza MS, Castro FLAL, Xue C, Zhang M, Qu J, Chlipala GE, Maienschein-Cline M, Silva TAD, Sousa SF, Dos Santos HT, Highlights of Tuft Cells in Mouse and Human Salivary Glands. Cells, 15(7):(2026) [pubmed]

10.3390/cells15070583;

submitter keyword: salivary gland,tuft cell, saliva, transcriptome and histology

Jun Qu
contact affiliation	Department of Pharmaceutical Sciences, University at Buffalo, The State University of New York, Buffalo, NY, USA
contact email	junqu@buffalo.edu
lab head
Chao Xue
contact affiliation	SUNY at Buffalo
contact email	chaoxue@buffalo.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD075581

PRIDE project URI

• PRIDE
  1. PXD075581
     1. Label: PRIDE project
     2. Name: Highlights of Tuft Cells in Mouse and Human Salivary Glands