PXD075566-1

PXD075566 is an original dataset announced via ProteomeXchange.

Title	2D-LC-MS/MS metaproteomes of the gutless marine worm Olavius algarvensis for the investigation of low-abundant symbionts
Description	Sulfate-reducing bacteria (SRB) are widespread in marine and terrestrial environments, where they often form syntrophic associations with bacteria, archaea, and eukaryotes. Among the most intimate of these are multipartite symbioses in gutless marine oligochaete worms, which host SRB and sulfur-oxidizing endosymbionts that exchange sulfur compounds syntrophically. To investigate this symbiosis, we generated 2D-LC-MS/MS metaproteomes of holobiont homogenates. Single Olavius algarvensis worms were pooled (n=50), homogenized and the symbionts were separated from each other and host debris by differential pelleting adapted from Hinzke et al., 2018. Homogenates were centrifuged for 2 minutes at 4 °C and 4000 x g (sample P1), the supernatant was transferred to a clean Eppendorf tube (sample S1), sterile filtered seawater (SFW) was added to sample P1; both P1 and S1 were centrifuged again with the same settings. The second supernatant from P1 was saved as S2 and again SFW was added to P1; S1 supernatant was transferred to a new tube (sample S3). P1 and S2 were centrifuged again for 2 minutes at 4 °C and 4000 x g. The resulting supernatant from P1 was discarded; the resulting supernatant from S2 was transferred to a new Eppendorf tube (sample S4). Samples S3 and S4 were centrifuged a final time for 7 minutes at 4 °C and 21000 x g and the resulting supernatant of S3 was saved in a new Eppendorf tube sample S5. Two of the resulting pellets (P1 most enriched in Cand. Thiosymbion, S3 most enriched in smaller symbionts (including Gamma3, Delta1, Delta4, Delta3, and the spirochete endosymbionts) and the final supernatant fraction (S5) were stored at -80 °C until further processing. Triplicates of the P1, S3, and S5 sample set were processed for proteomics, each resulting in 11 raw MS files corresponding to the different steps in the pH gradient. All 11 files were imported as sample fractions to Proteome Discoverer resulting in single results files for each sample.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_06:07:23.923.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Grace D'Angelo
SpeciesList	scientific name: Olavius algarvensis Delta 1 endosymbiont; NCBI TaxID: NEWT:334747; scientific name: Olavius algarvensis spirochete endosymbiont; NCBI TaxID: NEWT:260710; scientific name: Olavius algarvensis associated proteobacterium Delta 3; NCBI TaxID: NEWT:426688; scientific name: Olavius algarvensis; NCBI TaxID: NEWT:188229; scientific name: Olavius algarvensis Gamma 3 endosymbiont; NCBI TaxID: NEWT:260704; scientific name: Olavius algarvensis Gamma 1 endosymbiont; NCBI TaxID: NEWT:260705; scientific name: Olavius algarvensis Delta 4 endosymbiont; NCBI TaxID: NEWT:260707;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-12 10:25:53	ID requested
⏵ 1	2026-09-07 06:07:24	announced

10.1093/ISMEJO/WRAG190;

submitter keyword: symbiosis, gutless oligochaetes, metaproteome, chemoheterotrophs, microbiology, sulfate reducing bacteria,marine, metaproteomics

Manuel Kleiner
contact affiliation	Plant and Microbial Biology Department, North Carolina State University, USA
contact email	mkleine@ncsu.edu
lab head
Grace D'Angelo
contact affiliation	Max Planck Institute for Marine Microbiology
contact email	gdangelo@mpi-bremen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075566

PRIDE project URI

• PRIDE
  1. PXD075566
     1. Label: PRIDE project
     2. Name: 2D-LC-MS/MS metaproteomes of the gutless marine worm Olavius algarvensis for the investigation of low-abundant symbionts