PXD075533-1

PXD075533 is an original dataset announced via ProteomeXchange.

Title	A formate-based yeast chassis for efficient formate and CO₂ assimilation
Description	Formate is an attractive liquid carbon carrier that can be sustainably produced from carbon dioxide via electrochemical reduction, yet its utilization as the sole carbon source in eukaryotes is fundamentally constrained by inefficient energy coupling and severe stress toxicity. Here, we reprogrammed Yarrowia lipolytica into a synthetic formatotrophic yeast capable of stable growth on formate alone. Adaptive laboratory evolution yielded a strain with a biomass yield of 5.22 ± 0.12 g CDW mol⁻¹ formate, exceeding all previously reported formate-assimilating systems. Multi-omics and allelic reconstruction revealed a coupled adaptive program that redistributes ATP and reducing power, reinforces protein homeostasis via enhanced 26S proteasome assembly, and mitigates oxidative and carbonyl stress through coordinated membrane lipid remodeling and activation of the cellular integrity system. We further strengthened formate assimilation by rebalancing glycine-node flux partitioning, thereby maintaining high-throughput C1 delivery into central metabolism. Finally, integration of the evolved chassis with a copper–bismuth electrocatalyst in a near-neutral electrobiochemical system enabled the direct conversion of carbon dioxide and electrical energy into free fatty acids, establishing a mechanistically defined eukaryotic platform for formate-based CO₂ electrosynthesis
HostingRepository	iProX
AnnounceDate	2026-03-11
AnnouncementXML	Submission_2026-03-11_18:38:11.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yongmin Zheng
SpeciesList	scientific name: Yarrowia lipolytica; NCBI TaxID: 4952;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-11 18:37:42	ID requested
⏵ 1	2026-03-11 18:38:12	announced

Dataset with its publication pending

submitter keyword: yarrowia lipolytica, CO₂ assimilation, Formate

Wenming Zhang
contact affiliation	Nanjingtech University College of Biotechnology and Pharmaceutical
contact email	zhangwm@njtech.edu.cn
lab head
Yongmin Zheng
contact affiliation	Nanjingtech University College of Biotechnology and Pharmaceutical
contact email	2692308523@qq.com
dataset submitter

iProX dataset URI