PXD075456-1

PXD075456 is an original dataset announced via ProteomeXchange.

Title	A dedicated partitioning machinery ensures faithful inheritance of the apicoplast in T. gondii
Description	The tachyzoite stage of the apicomplexan parasite Toxoplasma gondii divides by an internal budding process called endodyogeny. Single copy organelles like the apicoplast, Golgi, and the mitochondrion require a precise machinery to ensure that each daughter cell receives exactly one organelle. The T. gondii apicoplast is partitioned between the two daughters by temporal association with the centrosomes, which is the major hub orchestrating the various cell division processes. However, the identity of the molecular infrastructure facilitating anchoring of the apicoplast to the centrosome is still unknown. In this study, we describe the apicoplast partitioning protein 1 (APP1), a hypothetical protein that first assembles in a specific basal complex (BC) section and then localizes to a region in close proximity to the apicoplast. Conditional APP1 knockdown resulted in a specific apicoplast partitioning defect. Reciprocally, upon apicoplast loss through ATG8-depletion, APP1 remains localized to the cytoskeleton, consolidating a role in apicoplast anchoring to the cell division infrastructure. Using APP1 as bait in a proximity biotinylation screen, we identified several known proteins of the inner membrane complex (IMC) and the BC, highlighting its close association with the parasite’s unique cytoskeleton. We further characterized proteins of unknown function and identified APP3, which positions APP1 to the specific site on the BC, but has overall a more pleiotropic role in coordinating cell division. In summary, APPs are functional connectors between the apicoplast and the daughter cytoskeleton scaffold (DCS) critical for apicoplast inheritance. Together our findings provide novel mechanistic insights into this unique aspect of apicomplexan organelle biology.
HostingRepository	PRIDE
AnnounceDate	2026-08-27
AnnouncementXML	Submission_2026-08-27_09:26:36.173.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Klemens Engelberg
SpeciesList	scientific name: Toxoplasma gondii RH; NCBI TaxID: NEWT:383379;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-10 12:11:10	ID requested
⏵ 1	2026-08-27 09:26:36	announced

Dataset with its publication pending

submitter keyword: Proximity Biotinylation, apicoplast, T. gondii

Dr. Marc-Jan Gubbels
contact affiliation	Boston College, Department of Biology, MA, USA
contact email	gubbelsj@bc.edu
lab head
Klemens Engelberg
contact affiliation	Boston College, Department of Biology
contact email	klemens.engelberg@bc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/08/PXD075456

PRIDE project URI

• PRIDE
  1. PXD075456
     1. Label: PRIDE project
     2. Name: A dedicated partitioning machinery ensures faithful inheritance of the apicoplast in T. gondii