⮝ Full datasets listing
PXD075442-1
PXD075442 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Analysis of the phosphorylation situation of CENP-U |
| Description | Study of the reconstituted in vitro human PLK1:CENP-U complex with PBD fused N-terminally to maltose-binding-protein (MBPPBD) and residues 58-114 of CENP-U fused N-terminally to MBP (MBPCENP-U58-114). To phosphorylate T78 and T98 of MBPCENP-U58-114, we added catalytic amounts (typically 1:30 molar ratio) of active CDK1 and PLK1 kinase. Either 1) pre-phosphorylating MBPCENP-U58-114 with both kinases before adding MBPPBD for complex formation (pre-phosphorylation method); or 2) mixing MBPCENP-U58-114 and MBPPBD with both kinases for simultaneous phosphorylation and complex formation (co-mixing method) were tested. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-03-11 |
| AnnouncementXML | Submission_2026-03-11_02:00:32.761.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Petra Janning |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | Phospho |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-10 06:21:48 | ID requested | |
| ⏵ 1 | 2026-03-11 02:00:33 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: phosphorylation, proteomics, CENP-U, DatasetType:Proteomics |
Contact List
| Petra Janning | |
|---|---|
| contact affiliation | MPI of Molecular Physiology |
| contact email | petra.janning@mpi-dortmund.mpg.de |
| lab head | |
| Petra Janning | |
| contact affiliation | MPI f�r molekulare Physiologie |
| contact email | petra.janning@mpi-dortmund.mpg.de |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v12/MSV000101084/ |
