PXD075416-1

PXD075416 is an original dataset announced via ProteomeXchange.

Title	Processing and release of the maize phytocytokine Zip1
Description	Phytocytokines are endogenous peptides that modulate plant immunity outcomes, yet how their maturation and spatial deployment are controlled remains unclear. Here we show that the maize phytocytokine precursor PROZIP1 is controlled by a spatially separated, two-stage proteolytic pathway that mechanistically uncouples signal activation from extracellular attenuation. PROZIP1 associates with the endoplasmic reticulum and undergoes intracellular, arginine-dependent processing by type II metacaspases, generating a C-terminal PROZIP1 fragment (Ct-PROZIP1). This processing licenses PROZIP1 for export to the apoplast via an ER-Golgi-independent route. Proteomic mapping and mutational analyses identify arginine residues flanking the Zip1 peptide as critical for efficient processing and secretion. The calcium-dependent metacaspase ZmMC9 specifically processes PROZIP1, thereby efficiently generating the bioactive Ct PROZIP1 fragment. In the apoplast, Ct-PROZIP1 is further processed by papain-like cysteine proteases and additional extracellular proteases, contributing to Zip1 turnover and signal clearance. While the free Zip1 peptide is detected at later stages, Ct-PROZIP appears to be the primary signaling entity in modulating pathogen-induced immune responses. Together, these findings demonstrate a previously unknown complexity in peptide signaling, suggesting a multilayered control of phytocytokine activity that provides spatial and temporal precision to disease modulation in maize.
HostingRepository	PRIDE
AnnounceDate	2026-08-04
AnnouncementXML	Submission_2026-08-04_02:21:21.963.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pitter Huesgen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	dimethylated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-09 15:45:53	ID requested
⏵ 1	2026-08-04 02:21:22	announced

10.1093/plphys/kiag533;

Koenig M, Sorger Z, Kakanj P, Dewes P, Mantz M, Perrar A, Sivaramakrishnan M, Stael S, Chandrasekar B, Huesgen PF, Villamil JM, Doehlemann G, Processing and release of the maize phytocytokine Zip1. Plant Physiol, 201(4):(2026) [pubmed]

submitter keyword: phytocytokine, metacaspase,Zip1, apoplastic immunity, PLCPs

Pitter F Huesgen
contact affiliation	Institute of Biology II, University of Freiburg, Freiburg, Germany Central Institute for Engineering, Electronics and Analytics, ZEA-3, Forschungzentrum Jülich, Jülich, Germany CIBSS-Centre for Integrative Biological Signaling Studies, University of Freiburg, Freiburg, Germany
contact email	pitter.huesgen@biologie.uni-freiburg.de
lab head
Pitter Huesgen
contact affiliation	University of Freiburg Faculty of Biology Biochemistry and Functional Proteomics
contact email	pitter.huesgen@biologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075416
     1. Label: PRIDE project
     2. Name: Processing and release of the maize phytocytokine Zip1