PXD075408 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants |
| Description | Recognition of dsRNA triggers antiviral defense mediated by PKR and OAS3/RNase L pathways through translational arrest and RNA decay. This is accompanied by assembly of distinct cytoplasmic ribonucleoprotein (RNP) condensates termed stress granules (SGs) and RNase L-dependent bodies (RLBs). Here we show that adenovirus infection differentially modulates dsRNA sensors and RNP granule assembly. Infection with splicing-defective ∆E4 mutant leads to dsRNA accumulation and activation of both PKR and OAS3/RNase L, promoting formation of RLB-like granules. In contrast, mutants lacking virus-associated (VA) RNAs trigger PKR activation and assembly of SGs despite absence of detectable dsRNA. Proteomic analysis revealed distinct protein compositions of canonical SGs and RLBs, which were reflected in virus-induced granules. While ∆VA-induced granules were PKR-dependent, ∆E4 mutants induced RLB-like granules independently of PKR and RNase L. In these cells, granule assembly coincided with translational arrest independent of eIF2α phosphorylation, indicating additional pathways linking nuclear dsRNA sensing to translational control and RNP granule assembly during viral infection. These findings provide novel insights into how distinct dsRNA sensors modulate translation and RNP condensates in response to stress. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-07-10 |
| AnnouncementXML | Submission_2026-07-10_11:58:34.528.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | ORLANDO SCUDERO |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-09 09:45:41 | ID requested | |
| ⏵ 1 | 2026-07-10 11:58:35 | announced | |
Publication List
Keyword List
| submitter keyword: DNA viruses, RNase L-dependent bodies (RLBs), Adenovirus 5 (Ad5),Stress Granules (SGs) |
Contact List
| Benjamin A. Garcia |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, Missouri, USA |
| contact email | bagarcia@wustl.edu |
| lab head | |
| ORLANDO SCUDERO |
|---|
| contact affiliation | Children's Hospital of Philadelphia |
| contact email | scuderoo@chop.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075408
- Label: PRIDE project
- Name: Differential assembly of RNP granules via activation of distinct dsRNA sensors by adenovirus mutants
