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PXD075381-1
PXD075381 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of BAR against placental dysfunction in RU486-induced abortion-prone mice |
| Description | Background Recurrent spontaneous abortion (RSA) remains a formidable clinical challenge with limited therapeutic options, largely attributed to trophoblast dysfunction as a central pathological mechanism. Bushen Antai recipe (BAR) has demonstrated encouraging clinical and experimental efficacy in pregnancy support; however, its bioactive constituents and molecular mechanisms, especially in the context of trophoblasts, remain poorly understood. Purpose To evaluate the therapeutic efficacy of BAR in RSA and elucidate its underlying mechanisms, with a particular focus on trophoblast function, using an integrated strategy. Methods UPLC-Q-TOF-MS/MS was employed to profile chemical constituents of BAR and its serum-absorbed components. An RU486-induced abortion-prone mouse model and HTR-8/SVneo trophoblast injury model were established to evaluate BAR's effects on embryonic development and placentation. Label-free quantitative proteomics, network pharmacology, molecular docking, viral transduction, together with transmission electron microscopy imaging, morphological analysis, western blot, immunofluorescence, and various cellular functional assays were performed to decipher the underlying mechanisms. Results BAR significantly reduced embryo resorption, restored placental architecture, and improved trophoblast survival and function in abortion-prone mice. Proteomics revealed endoplasmic reticulum (ER) stress as a central pathological feature of RSA placentas, which was effectively ameliorated by BAR treatment. Network pharmacology predicted VEGFA as a core target, subsequently validated by experiments demonstrating that BAR activates the VEGFA/p-AKT/SESN2 axis to reprogram the dynamics of all three unfolded protein response (UPR) branches and re-establish ER homeostasis. Serum pharmacochemistry identified 72 absorbed constituents, with molecular docking revealing that multiple compounds including quercetin, calycosin, loganin, and sweroside may directly target VEGFA. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-07 |
| AnnouncementXML | Submission_2026-03-09_01:38:11.110.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Zhuo Liu |
| SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF-X |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-09 01:37:57 | ID requested | |
| ⏵ 1 | 2026-03-09 01:38:11 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Recurrent spontaneous abortion, Bushen Antai recipe,Trophoblasts, Endoplasmic reticulum stress |
Contact List
| Yufan Song | |
|---|---|
| contact affiliation | Department of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology |
| contact email | songyufan23@163.com |
| lab head | |
| Zhuo Liu | |
| contact affiliation | Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology |
| contact email | zhuoliutjzx@126.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
