PXD075371 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva |
| Description | Chlorophycean algae of the genera Chlamydomonas and Polytomella share a common photosynthetic ancestor. However, members of the Polytomella lineage have adopted a heterotrophic lifestyle, having lost the photosynthetic apparatus and relying instead on acetate or ethanol as carbon sources, with energy production centered on oxidative phosphorylation (OXPHOS). In this study, we investigated the composition of the mitochondrial supercomplexes of the colorless alga Polytomella parva. Mitochondrial membranes were solubilized using mild detergents such as glycol-diosgenin and digitonin, followed by separation of OXPHOS complexes supramolecular assemblies via Blue Native electrophoresis and Fast Protein Liquid Chromatography (FPLC). Additionally, complexome profiling of solubilized algal mitochondria resolved by Blue Native Gel Electrophoresis was carried out. The resulting data indicate that the OXPHOS supercomplexes of Polytomella closely resemble those observed in situ in the mitochondria of its green relative Chlamydomonas reinhardtii, as revealed by electron cryo-tomography and subtomogram averaging. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-06-01 |
| AnnouncementXML | Submission_2026-05-31_16:09:20.023.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Alfredo Cabrera-Orefice |
| SpeciesList | scientific name: Polytomella parva; NCBI TaxID: NEWT:51329; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-08 23:59:11 | ID requested | |
| ⏵ 1 | 2026-05-31 16:09:20 | announced | |
Publication List
| 10.1007/s10863-026-10103-3; |
| Ostolga-Chavarr, í, a M, Rico-Luna A, Fuentes-Hern, á, ndez S, Miranda-Astudillo HV, Cabrera-Orefice A, Gonz, á, lez-Halphen D, Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva. J Bioenerg Biomembr, 58(1):(2026) [pubmed] |
Keyword List
| submitter keyword: oxidative phosphorylation, F1FO ATP synthase, mitochondrial supercomplexes, oligomeric complex V,Chlorophycean algae |
Contact List
| Prof. Dr. Diego González-Halphen |
|---|
| contact affiliation | Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico. |
| contact email | dhalphen@ifc.unam.mx |
| lab head | |
| Alfredo Cabrera-Orefice |
|---|
| contact affiliation | Institute of Biochemistry, Justus-Liebig-University Giessen |
| contact email | alfredbiomed@gmail.com |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075371
- Label: PRIDE project
- Name: Mitochondrial super complexes in the colorless chlorophycean alga Polytomella parva
