PXD075370 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Glycoproteome Profiling of Human Serum for Hepatocellular Carcinoma Biomarker Discovery |
| Description | Hepatocellular carcinoma (HCC) typically develops on a cirrhotic background, where conventional serum biomarkers such as alpha-fetoprotein (AFP) provide inconsistent performance. We implemented an integrated serum intact glycoproteomics strategy that profiles N-linked and O-linked glycopeptides in parallel. This strategy couples prevalence-aware filtering with multi-algorithm feature selection to prioritize glycoform-resolved markers distinguishing HCC from cirrhosis. Serum from HCC (n = 20) and cirrhosis (n = 20) underwent high-abundance protein depletion, proteolysis, glycopeptide enrichment, and high-resolution LC–MS/MS. Intact glycopeptides were identified with Byonic/Proteome Discoverer and filtered to a high-confidence quantitative matrix requiring ≥70% feature presence. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-09-07 |
| AnnouncementXML | Submission_2026-09-07_04:20:55.863.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Rency Varghese |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2026-03-08 22:31:42 | ID requested | |
| ⏵ 1 | 2026-09-07 04:20:56 | announced | |
Publication List
| 10.1021/acs.jproteome.6c00258; |
| Sajid MS, Varghese RS, Saleem S, Zhang X, Kroemer A, Ressom HW, Glycoproteome Profiling of Human Serum for Hepatocellular Carcinoma Biomarker Discovery. J Proteome Res, 25(7):3705-3715(2026) [pubmed] |
Keyword List
| submitter keyword: nano-LC-MS/MS,Liver cancer, glycoproteomics, human Serum, biomarker |
Contact List
| Habtom Ressom |
|---|
| contact affiliation | Georgetown University Medical Center |
| contact email | hwr@georgetown.edu |
| lab head | |
| Rency Varghese |
|---|
| contact affiliation | Georgetown University |
| contact email | rsv4@georgetown.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075370 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD075370
- Label: PRIDE project
- Name: Glycoproteome Profiling of Human Serum for Hepatocellular Carcinoma Biomarker Discovery
