PXD075265-1

PXD075265 is an original dataset announced via ProteomeXchange.

Title	Uridine restores oocyte quality and mitigates female reproductive aging by inhibition of ferroptosis in mice
Description	Advanced maternal age is a key factor in female infertility, primarily due to declines in ovarian reserve and oocyte quality. However, the metabolic mechanisms underlying reproductive aging remain unclear. Here, we show that uridine levels in the plasma and ovaries of aged mice are significantly reduced compared with young controls. Building on this, we find that uridine supplementation significantly improves meiotic maturation, fertilization, and early embryonic development of aged oocytes, both in vivo and in vitro. Further microtranscriptomic analyses reveal that uridine enhances oocyte quality by inhibiting ferroptosis and enhancing mitochondrial function. Moreover, integrating Limited Proteolysis-Small Molecule Mapping, western blot and siRNA-based functional assays, we identify that uridine binds Poly(rC)-binding protein 1, thereby suppressing ferroptosis and preserving mitochondrial function. Collectively, these findings demonstrate that uridine supplementation improves fertility in aged female mice and provide mechanistic insight into ferroptosis in oocyte aging.
HostingRepository	PRIDE
AnnounceDate	2026-03-05
AnnouncementXML	Submission_2026-03-05_06:11:31.894.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JING CHEN
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	6220 Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-05 03:54:01	ID requested
⏵ 1	2026-03-05 06:11:32	announced

Dataset with its publication pending

submitter keyword: PCBP1, ferroptosis, uridine, oocyte quality,Advanced maternal age

Jingyue Chen
contact affiliation	Peking University Third Hospital No. 49 North Garden Road, Haidian District Beijing 100191, China
contact email	2416397026@bjmu.edu.cn
lab head
JING CHEN
contact affiliation	peking university third hospital
contact email	2416397026@bjmu.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD075265
     1. Label: PRIDE project
     2. Name: Uridine restores oocyte quality and mitigates female reproductive aging by inhibition of ferroptosis in mice