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PXD075262-1
PXD075262 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of Bifemetstrobin against the tea anthracnose pathogen |
| Description | Tea anthracnose caused by Glomerella cingulata threatens yield and quality, while resistance to legacy fungicides highlights the need for new modes of action. We characterized the antifungal activity and mechanism of bifemetstrobin against G. cingulata by combining efficacy assays with cell biology and integrated multi‑omics. Bifemetstrobin inhibited mycelial growth (EC50 = 5.04 μg/mL) and spore germination and provided up to approximately 80% curative suppression on detached tea leaves. Microscopy revealed dose‑dependent hyphal and conidial deformation and mitochondrial injury. Consistently, bifemetstrobin collapsed mitochondrial membrane potential (MMP), reduced intracellular ATP, and increased membrane leakage with depletion of reducing sugars and soluble proteins. Transcriptome, data-independent acquisition (DIA) proteomics and metabolome data converged on reprogramming of carbohydrate and amino‑acid metabolism, membrane processes, and redox homeostasis, with DNA‑replication signatures emerging at the higher dose. Cross-layer mapping prioritized an amine oxidase (AO) as a hub. Bifemetstrobin bound AO with micromolar affinity (microscale thermophoresis/isothermal titration calorimetry), supported by docking and molecular dynamics, and outperformed azoxystrobin in binding. Pairing bifemetstrobin with pyrrolnitrin yielded strong in vitro synergy and improved leaf protection. Across additional phytopathogens, bifemetstrobin retained activity in vitro (EC50 range 1.19–27.38 μg/mL), outperforming azoxystrobin on the tea anthracnose strain. These findings nominate AO as a high‑confidence candidate supported by binding assays and link target engagement to mitochondrial failure and carbon-nitrogen reprogramming, supporting bifemetstrobin (alone or in rational mixtures) as a promising candidate for tea anthracnose management. |
| HostingRepository | iProX |
| AnnounceDate | 2026-03-05 |
| AnnouncementXML | Submission_2026-03-05_01:11:45.930.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Xinpeng Zhang |
| SpeciesList | scientific name: Colletotrichum gloeosporioides f. sp. camelliae; NCBI TaxID: 1215364; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Astral |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2026-03-05 01:11:26 | ID requested | |
| ⏵ 1 | 2026-03-05 01:11:46 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Proteomic, Bifemetstrobin |
Contact List
| Xinpeng Zhang | |
|---|---|
| contact affiliation | Guizhou University |
| contact email | zhangxinpeng175@163.com |
| lab head | |
| Xinpeng Zhang | |
| contact affiliation | Guizhou University |
| contact email | zhangxinpeng175@163.com |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
