PXD075256-1

PXD075256 is an original dataset announced via ProteomeXchange.

Title	The life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera)
Description	Deleterious mutations arise continually in natural populations, yet their full life histories—from origin to elimination—are rarely observed directly. Most understanding of mutational fate in the wild comes from indirect inference rather than from tracking a single mutation across generations. Haplodiploid systems provide a rare opportunity to observe selection in action: because males are haploid, recessive alleles are immediately exposed to selection rather than masked in heterozygotes. This genetic architecture has long been hypothesized to accelerate the purging of deleterious variation. Here, we leverage the honey bee to document the complete trajectory of a spontaneous deleterious mutation, from its origin in a single queen to its extinction. The mutation alters eye pigmentation in haploid males, enabling us to quantify its molecular, physiological, and behavioral consequences through integrated genomic, transcriptomic, proteomic, and metabolomic analyses. By following this mutation across generations, we directly observe how haplodiploidy exposes recessive alleles to immediate purifying selection, and how mating structure and colony demography influence their persistence. This study represents one of the few documented cases of a naturally occurring mutation tracked through its full evolutionary course, offering a rare empirical view of how selection operates in real time within a social, haplodiploid genome.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-07_04:20:39.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jenny Moon
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: NEWT:7460;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2026-03-04 22:40:50	ID requested
⏵ 1	2026-09-07 04:20:40	announced

10.1093/molbev/msag156;

Shultz RR, Nixon JM, Ryals DK, Buschkoetter AC, Gilchrist IR, Given JK, Metz BN, Maga, ñ, a AA, Moon KM, Thanepohn B, Mahood J, Tarpy DR, Foster LJ, Harpur BA, The life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera). Mol Biol Evol, 43(7):(2026) [pubmed]

submitter keyword: Eye mutation, ivory mutation, cinnabar, Hymenoptera, genome-wide association study, recurrent mutation

Leonard J. Foster
contact affiliation	Michael Smith Laboratories, Biochemistry and Molecular Biology, University of British Columbia, Canada
contact email	foster@msl.ubc.ca
lab head
Jenny Moon
contact affiliation	University of British Columbia
contact email	kyungmee@mail.ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/09/PXD075256

PRIDE project URI

• PRIDE
  1. PXD075256
     1. Label: PRIDE project
     2. Name: The life history of recessive deleterious alleles as seen through the eyes of a honey bee (Apis mellifera)